spp. isolated from human and environmental samples in Spain: detection of 11 new species.

One hundred thirty-six isolates, 88 human and 48 environmental, that met the requirements to belong to the genus were identified using a polyphasic taxonomic approach known as 16S rRNA plus phenotypic traits. Thirty-seven species were identified; some had not been previously reported from clinical samples. The main species were (13 isolates), (11), (9) and (8). (11/13) and (8/11) were mainly environmental isolates, while (9/9) and (6/8) were mainly human isolates. Despite the difficulties in assigning to human isolates a role as a pathogen or contaminant, here 25% of the isolates were involved in true infections, especially in those cases that affected abscesses, wound exudates, ocular infections and diverse fluids. In addition, 15 isolates were identified as 11 to a new species, all of them from human specimens except one that was obtained from laboratory air The antimicrobial susceptibility testing showed 95.6% of isolates were resistant to ampicillin, 44% were resistant to cotrimoxazole, 20 to 30% were resistant to cefotaxime and vancomycin and 13% were resistant to rifampicin and erythromycin.