Gholipour Amir Reza, Jafari Leila, Ramezanpour Mahsa, Evazalipour Mehdi, Chavoshi Maral, Yousefbeyk Fatemeh, Kargar Moghaddam Saghi Jani, Yekta Kooshali Mohammad Hossein, Ramezanpour Nahid, Daei Puyan, Ghasemi Saeed, Hamidi Masoud
Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Pediatric Cell and Gene Therapy Research Center, Gene, Cell and Tissue Research Institute, Tehran University of Medical Science Tehran, Iran.
Galen Med J. 2022 Nov 16;11:e2484. doi: 10.31661/gmj.v11i.2484. eCollection 2022.
Recently, the non-toxic properties of natural plant products have gained more focus as anticancer agents. Therefore, this study aimed to assess the apoptosis effects of the ethanolic extract of on the MCF-7 breast cancer cell line. In this experimental study, aerial parts of were collected in Lahijan city (Iran), and after confirmation, they were dried and extracted with ethanol for 24 h. Then, the total phenolic and flavonoid contents of the extract were measured. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was used to measure the antioxidant properties of the extract. Selected cell lines (MCF-7 and human dermal fibroblast) were cultured in 6-wells dishes (1×10 cells/well). After 72 h of treating the extract, cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of apoptotic genes (such as , , , and ) was studied by real-time polymerase chain reaction (PCR). The extract's total phenolic content was 31.30±02 μg of gallic acid equivalents/mg of dry extract, and the total flavonoid content was 49.61±04 μg of quercetin as equivalents/mg of extract. The antioxidant activity of was measured at the dose of 619.2 μg/μl, indicating that it decreases cancer cell viability and enhances apoptosis. Within the half maximal inhibitory concentrations, real-time PCR revealed substantial increases in (P<0.001), (P<0.05), and expression (P<0.05) in MCF-7 cells treated with . This study suggests that may cause apoptosis by oxidative stress in cancer cells.[GMJ.2022;11:e2484].
最近，天然植物产品的无毒特性作为抗癌剂受到了更多关注。因此，本研究旨在评估[植物名称未给出]乙醇提取物对MCF-7乳腺癌细胞系的凋亡作用。在这项实验研究中，[植物名称未给出]的地上部分在伊朗拉希詹市采集，经确认后干燥并用乙醇提取24小时。然后，测定提取物的总酚和黄酮含量。采用2,2-二苯基-1-苦基肼自由基清除试验测定提取物的抗氧化性能。将选定的细胞系（MCF-7和人皮肤成纤维细胞）接种于6孔板（1×10个细胞/孔）。用提取物处理72小时后，使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴盐（MTT）试验评估细胞毒性。通过实时聚合酶链反应（PCR）研究凋亡基因（如[基因名称未给出]、[基因名称未给出]、[基因名称未给出]和[基因名称未给出]）的表达。提取物的总酚含量为31.30±02微克没食子酸当量/毫克干提取物，总黄酮含量为49.61±04微克槲皮素当量/毫克提取物。[植物名称未给出]在619.2微克/微升剂量下的抗氧化活性表明，它可降低癌细胞活力并增强凋亡。在半数最大抑制浓度范围内，实时PCR显示用[植物名称未给出]处理的MCF-7细胞中[基因名称未给出]（P<0.001）、[基因名称未给出]（P<0.05）和[基因名称未给出]表达（P<0.05）大幅增加。本研究表明，[植物名称未给出]可能通过癌细胞中的氧化应激导致凋亡。[《胃肠病学与肝病杂志》.2022;11:e2484]
