Rational synthesis, biological screening of azo derivatives of chloro-phenylcarbonyl diazenyl hydroxy dipyrimidines/thioxotetrahydropyrimidines and their metal complexes.

Herein, a new series of azo ligands HL-1 , HL-2 HL-3 HL-4 and their metal complexes with Cu(II) & Ni(II) were synthesized successfully having excellent yield, in reproducible conditions and for structure elucidation different advance spectroscopic techniques (FTIR, H NMR, C NMR and Mass Spectrometry) were applied. In FTIR analysis, the absence of peak at due to -NH and presence of a new peak of N=N at 1390-1520 cm confirmed synthesis of the ligands. The H NMR spectra of azo ligands showed singlet peak at 11.5-13.5 ppm (Ar-OH) for hydroxyl group and -NH signals disappearance of anilines at 4-5 ppm also gives strong indication for the synthesis of azo compounds. On complexation two most important peaks (M-O, M-N) appeared in all the metal chelates in the range of 400-600 cm which were not present in any of the ligands, confirmed the formation of complexes. Molecular ion peaks in mass spectra at 273, 388, 407 and 423 m/z value for ligands as well as for complexes at 803, 835, 871 and 904 m/z also give strong indication that proposed ligands and their metal complexes are produced successfully. Biological screening of the synthesized compounds were also carried out against different bacterial strains (, , and , antifungal (., , and strains and antioxidant activity. From results it was observed that HL-4 and Cu complexes exhibited maximum inhibition against all bacterial and fungal strains as compared to other ligands and standard drug.