The detection of multiple analytes by using visual colorimetric and fluorometric multimodal chemosensor based on the azo dye.

In recent decades, researchers have conducted in-depth studies of the design and synthesis of colorimetric/fluorometric probes and the application of such probes to biological and practical samples. The multifunctional colorimetric and fluorescent azo benzene-based probe (4'-hydroxyl-2,4-diaminoazobenzene, ) was designed to detect Al, Fe, Cu and F¯. Based on the distinct redshift of the absorption band and a significant color change (yellow → purple), was utilized for both naked-eyed and quantitative detection of Al and Fe after formation of the 1:1 complex. Test paper coated with and used in conjunction with a cell phone was used for colorimetric detection of Al and Fe ions (20 μM-2.0 mM) in water samples through naked-eye and digital image colorimetry. The "-Fe" coordination shift that occurs in the presence of the competitive ligand F¯ was used in the colorimetric measurement of F¯ in toothpaste. In the presence of Cu ion, the non-emissive has transformed into fluorescent benzotriazole product (Φ = 0.53) through the bimolecular rate-limiting step, and the second-order rate constant is calculated as 31 ± 2 M s. exhibits a "turn-on" fluorescence response in the presence of Cu that is greater than its response in the presence of competitive species such as Fe, Al, Co, Fe, Zn, Cd, Mg, Mn, Ni and Ag. was shown to have low toxicity to living HeLa cells and to present good imaging characteristics for tracking of Cu in vivo.