Ren Hong, Li Fei, Yu Shihua, Wu Ping
School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin City, 132022, PR China.
Heliyon. 2022 Aug 13;8(8):e10216. doi: 10.1016/j.heliyon.2022.e10216. eCollection 2022 Aug.
In recent decades, researchers have conducted in-depth studies of the design and synthesis of colorimetric/fluorometric probes and the application of such probes to biological and practical samples. The multifunctional colorimetric and fluorescent azo benzene-based probe (4'-hydroxyl-2,4-diaminoazobenzene, ) was designed to detect Al, Fe, Cu and F¯. Based on the distinct redshift of the absorption band and a significant color change (yellow → purple), was utilized for both naked-eyed and quantitative detection of Al and Fe after formation of the 1:1 complex. Test paper coated with and used in conjunction with a cell phone was used for colorimetric detection of Al and Fe ions (20 μM-2.0 mM) in water samples through naked-eye and digital image colorimetry. The "-Fe" coordination shift that occurs in the presence of the competitive ligand F¯ was used in the colorimetric measurement of F¯ in toothpaste. In the presence of Cu ion, the non-emissive has transformed into fluorescent benzotriazole product (Φ = 0.53) through the bimolecular rate-limiting step, and the second-order rate constant is calculated as 31 ± 2 M s. exhibits a "turn-on" fluorescence response in the presence of Cu that is greater than its response in the presence of competitive species such as Fe, Al, Co, Fe, Zn, Cd, Mg, Mn, Ni and Ag. was shown to have low toxicity to living HeLa cells and to present good imaging characteristics for tracking of Cu in vivo.
近几十年来，研究人员对比色/荧光探针的设计与合成及其在生物和实际样品中的应用进行了深入研究。设计了多功能的基于偶氮苯的比色和荧光探针（4'-羟基-2,4-二氨基偶氮苯）来检测铝、铁、铜和氟离子。基于吸收带明显的红移和显著的颜色变化（黄色→紫色），该探针在形成1:1配合物后用于铝和铁的裸眼检测和定量检测。涂有该探针并与手机配合使用的试纸，通过裸眼和数字图像比色法用于水样中铝和铁离子（20 μM - 2.0 mM）的比色检测。在竞争性配体氟离子存在下发生的“-Fe”配位位移用于牙膏中氟离子的比色测定。在铜离子存在下，无发射的该探针通过双分子限速步骤转化为荧光苯并三唑产物（荧光量子产率Φ = 0.53），二阶速率常数计算为31 ± 2 M⁻¹ s⁻¹。该探针在铜存在下表现出“开启”荧光响应，且大于其在铁、铝、钴、铁、锌、镉、镁、锰、镍和银等竞争物种存在下的响应。结果表明该探针对活的HeLa细胞毒性低，并且在体内追踪铜时具有良好的成像特性。
