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植物油中凝集素的分离与定量分析。

Isolation and quantitation of lectins from vegetable oils.

作者信息

Klurfeld D M, Kritchevsky D

机构信息

Wistar Institute, Philadelphia, PA 19104.

出版信息

Lipids. 1987 Sep;22(9):667-8. doi: 10.1007/BF02533947.

DOI:10.1007/BF02533947
PMID:3669929
Abstract

The factor(s) responsible for the unexplained atherogenicity of peanut oil remain to be elucidated. To this end, we developed a technique to determine if lectin was present in the oil and to quantitate its concentration. This technique was applied to other vegetable oils including corn, soybean, and sunflower. Crude, unprocessed corn and soybean oils were also analyzed for lectin content. The crude oils contained from 858 to 2983 micrograms lectin per kg, while the refined oils contained 24 to 55 micrograms/kg of biologically active lectin. The identities of the isolated lectins were confirmed by electrophoresis on SDS-polyacrylamide gels. The biological significance of the presence of lectin in these oils remains to be determined.

摘要

花生油产生无法解释的致动脉粥样化作用的因素仍有待阐明。为此,我们开发了一种技术来确定油中是否存在凝集素并对其浓度进行定量。该技术应用于其他植物油,包括玉米油、大豆油和向日葵油。还对未加工的粗制玉米油和大豆油的凝集素含量进行了分析。粗制油中每千克含有858至2983微克凝集素,而精炼油中每千克含有24至55微克具有生物活性的凝集素。通过在SDS-聚丙烯酰胺凝胶上进行电泳确认了分离出的凝集素的身份。这些油中存在凝集素的生物学意义仍有待确定。

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Isolation and quantitation of lectins from vegetable oils.植物油中凝集素的分离与定量分析。
Lipids. 1987 Sep;22(9):667-8. doi: 10.1007/BF02533947.
2
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引用本文的文献

1
Lectin may contribute to the atherogenicity of peanut oil.凝集素可能会导致花生油的致动脉粥样化性。
Lipids. 1998 Aug;33(8):821-3. doi: 10.1007/s11745-998-0276-9.
2
Atherogenic potential of peanut oil-based monounsaturated fatty acids diets.花生油基单不饱和脂肪酸饮食的致动脉粥样硬化潜力。
Lipids. 1998 Feb;33(2):229-30. doi: 10.1007/s11745-998-0200-3.

本文引用的文献

1
Cholesterol vehicle in experimental atherosclerosis. Part 18. Comparison of North American, African and South American peanut oils.实验性动脉粥样硬化中的胆固醇载体。第18部分。北美、非洲和南美花生油的比较。
Atherosclerosis. 1981 Feb-Mar;38(3-4):291-9. doi: 10.1016/0021-9150(81)90045-9.
2
Influence of native and randomized peanut oil on lipid metabolism and aortic sudanophilia in the vervet monkey.天然花生油和随机化花生油对绿猴脂质代谢及主动脉苏丹ophilia的影响。
Atherosclerosis. 1982 Mar;42(1):53-8. doi: 10.1016/0021-9150(82)90125-3.
3
Lectins in the United States diet: a survey of lectins in commonly consumed foods and a review of the literature.
美国饮食中的凝集素:常见食用食物中凝集素的调查及文献综述。
Am J Clin Nutr. 1980 Nov;33(11):2338-45. doi: 10.1093/ajcn/33.11.2338.
4
The absorption and transport of dietary cholesterol in the presence of peanut oil or randomized peanut oil.在花生油或随机化花生油存在的情况下膳食胆固醇的吸收与转运
Lipids. 1984 Jan;19(1):11-6. doi: 10.1007/BF02534602.
5
In vitro immune aggression against rabbit aortic smooth muscle cells.
Atherosclerosis. 1984 May-Jun;51(2-3):269-80. doi: 10.1016/0021-9150(84)90174-6.
6
Experimental atherosclerosis in rabbits fed cholesterol-free diets. Part 12. Comparison of peanut and olive oils.给家兔喂食不含胆固醇的饲料引发的实验性动脉粥样硬化。第12部分。花生油与橄榄油的比较。
Atherosclerosis. 1984 Mar;50(3):253-9. doi: 10.1016/0021-9150(84)90073-x.
7
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
8
Specific inhibition by N-acetyl-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces.N-乙酰-D-半乳糖胺对大豆凝集素与动物细胞表面相互作用的特异性抑制作用。
Biochim Biophys Acta. 1970 Sep 15;211(3):582-5. doi: 10.1016/0005-2736(70)90265-8.
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Ultrastructural localization of monosaccharide residues on cerebral endothelium.大脑内皮细胞上单糖残基的超微结构定位
Lab Invest. 1985 May;52(5):553-8.
10
Detection of soy protein in soy lecithin, margarine and, occasionally, soy oil.在大豆卵磷脂、人造黄油以及偶尔在大豆油中检测大豆蛋白。
Int Arch Allergy Appl Immunol. 1985;78(1):30-2. doi: 10.1159/000233858.