Tso P, Pinkston G, Klurfeld D M, Kritchevsky D
Lipids. 1984 Jan;19(1):11-6. doi: 10.1007/BF02534602.
Peanut oil has been shown to be unexpectedly atherogenic for cholesterol-fed rats, rabbits and rhesus monkeys. However, randomization (rearrangement of fatty acids to random distribution) of peanut oil significantly reduced its atherogenicity for rabbits and monkeys. This study was conducted to investigate whether the absorption and transport of dietary cholesterol was altered in the presence of peanut oil or randomized peanut oil, thereby accounting for the difference in the atherogenicity of the two diets. Intestinal lymph fistula rats were infused intraduodenally with a lipid emulsion at a rate of 3 ml/hr. The lipid emulsion contained either peanut oil (control) or randomized peanut oil (experimental) (10 mM), 14C-cholesterol (1.3 mM) and sodium taurocholate (19 mM) in phosphate-buffered saline, pH 6.4. Lymph triglyceride, cholesterol and phospholipid outputs were similar in both groups of rats during fasting and subsequently during lipid infusion. Comparable recovery of 14C-cholesterol from the intestinal lumen and the intestinal mucosa of the control and the experimental rats showed that the absorption and transport of dietary cholesterol were similar in both groups of rats. Analyses of the fatty acid of both lymph and intestinal mucosal lipid again failed to reveal a difference between the 2 groups of rats. It is concluded that the difference in the atherogenicity between the peanut oil and the randomized peanut oil is probably caused by events subsequent to the release of cholesterol containing chylomicrons and very low density lipoproteins by the small intestinal epithelial cells.
花生油已被证明对喂食胆固醇的大鼠、兔子和恒河猴具有意想不到的致动脉粥样硬化作用。然而，花生油的随机化（脂肪酸重排至随机分布）显著降低了其对兔子和猴子的致动脉粥样硬化性。本研究旨在调查在存在花生油或随机化花生油的情况下，膳食胆固醇的吸收和转运是否发生改变，从而解释两种饮食致动脉粥样硬化性的差异。给肠淋巴瘘大鼠以3 ml/小时的速率十二指肠内输注脂质乳剂。脂质乳剂在pH 6.4的磷酸盐缓冲盐水中含有花生油（对照）或随机化花生油（实验）（10 mM）、14C-胆固醇（1.3 mM）和牛磺胆酸钠（19 mM）。两组大鼠在禁食期间以及随后脂质输注期间的淋巴甘油三酯、胆固醇和磷脂输出相似。对照大鼠和实验大鼠从肠腔和肠黏膜中14C-胆固醇的可比回收率表明，两组大鼠膳食胆固醇的吸收和转运相似。对淋巴和肠黏膜脂质脂肪酸的分析再次未能揭示两组大鼠之间的差异。得出的结论是，花生油和随机化花生油之间致动脉粥样硬化性的差异可能是由小肠上皮细胞释放含乳糜微粒和极低密度脂蛋白的胆固醇之后的事件引起的。
