从不同年龄组大鼠中获得的肝酸性胆固醇酯水解酶的活性和特性。

The activity and properties of a hepatic acid cholesteryl ester hydrolase obtained from rats of different age groups.

作者信息

Tanaka M, Iio T, Tabata T

机构信息

Showa College of Pharmaceutical Sciences, Tokyo, Japan.

出版信息

Lipids. 1987 Sep;22(9):677-9. doi: 10.1007/BF02533950.

DOI:10.1007/BF02533950

PMID:3669930

Abstract

The activity of lysosomal acid cholesteryl ester hydrolase (acid CEH, EC 3.1.1.13) in rat liver was determined at 3, 5, 7, 10 and 20 wk following birth. The levels of acid CEH activity showed a marked decrease as rats grew older, whereas those of other lysosomal marker enzymes, such as acid phosphatase, beta-glucuronidase and cathepsin B and D, showed only a slight decrease. On the other hand, acid CEH activity was detected in all subcellular fractions obtained from rat liver, but the enzyme activity in these fractions did not show the age-related decrease observed in the lysosomal fraction. The results presented here suggest that the marked alteration of lysosomal acid CEH activity that accompanies aging may be related to its possible involvement in the regulation of cholesterol concentration in rat liver.

摘要

测定了出生后3、5、7、10和20周龄大鼠肝脏中溶酶体酸性胆固醇酯水解酶（酸性CEH，EC 3.1.1.13）的活性。随着大鼠年龄的增长，酸性CEH活性水平显著下降，而其他溶酶体标记酶，如酸性磷酸酶、β-葡萄糖醛酸酶、组织蛋白酶B和D的活性水平仅略有下降。另一方面，在从大鼠肝脏获得的所有亚细胞组分中均检测到酸性CEH活性，但这些组分中的酶活性并未表现出溶酶体组分中观察到的与年龄相关的下降。本文给出的结果表明，衰老过程中溶酶体酸性CEH活性的显著改变可能与其参与大鼠肝脏胆固醇浓度的调节有关。