Tanaka M, Yonekura R, Iio T, Tabata T
Lipids. 1984 Oct;19(10):714-20. doi: 10.1007/BF02534464.
An inhibitor of lysosomal acid cholesteryl ester hydrolase (Acid CEH), (EC 3.1.1.13) was found in the cytosolic fraction of rat liver and various other tissues. The extent of the inhibitory effect was dependent on the concentration of the cytosolic protein. The Acid CEH inhibitor was heat-labile, non-dialyzable, and its inhibitory activity significantly decreased by trypsin or chymotrypsin digestion, but not by lipase digestion. The inhibitor had no effect on the activity of cathepsin D, beta-glucuronidase and acid phosphatase, which are other enzymes found in lysosomes. The present findings suggest that the inhibitor may be involved in the regulation of the hydrolysis of cholesteryl esters in lipoproteins that have been transferred into the liver.
在大鼠肝脏及其他多种组织的胞质组分中发现了一种溶酶体酸性胆固醇酯水解酶（酸性CEH，EC 3.1.1.13）抑制剂。抑制作用的程度取决于胞质蛋白的浓度。该酸性CEH抑制剂对热不稳定，不可透析，其抑制活性经胰蛋白酶或糜蛋白酶消化后显著降低，但经脂肪酶消化则无影响。该抑制剂对组织蛋白酶D、β-葡萄糖醛酸酶和酸性磷酸酶（这些是溶酶体中发现的其他酶）的活性没有影响。目前的研究结果表明，该抑制剂可能参与了转运至肝脏的脂蛋白中胆固醇酯水解的调节。
