Cui Chunmei, Fan Rui, Zhou Yuan, Cui Qinghua
Department of Biomedical Informatics, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
Bioinform Adv. 2021 Dec 7;2(1):vbab040. doi: 10.1093/bioadv/vbab040. eCollection 2022.
It is well-known that some microRNAs (miRNAs) are more important than the others for life, hinting the wide range of miRNA in essentiality or importance. Functional enrichment analysis is a quite pervasive method to dig out the underlying biological pathway for a given gene list and several tools of miRNA set enrichment analysis have been developed. However, all those tools treat each miRNA equally and neglect the importance score of miRNA itself, which could be an obstacle to seek more insightful biological processes for researchers. Here, we developed wTAM, a tool for annotation of weighted human miRNAs, introducing the miRNA importance scores into enrichment analysis. In addition, the annotation repository has been enlarged comparing to TAM. Finally, the case study demonstrated the availability and flexibility of wTAM.
wTAM is freely available at http://www.cuilab.cn/wtam/.
Supplementary data are available at online.
众所周知，某些微小RNA（miRNA）对生命而言比其他的更为重要，这暗示了miRNA在重要性或关键性方面具有广泛差异。功能富集分析是一种非常普遍的方法，用于挖掘给定基因列表潜在的生物学途径，并且已经开发了几种miRNA集富集分析工具。然而，所有这些工具对每个miRNA一视同仁，忽略了miRNA本身的重要性评分，这可能会成为研究人员寻找更具洞察力的生物学过程的障碍。在此，我们开发了wTAM，一种用于加权人类miRNA注释的工具，将miRNA重要性评分引入富集分析。此外，与TAM相比，注释库已得到扩充。最后，案例研究证明了wTAM的实用性和灵活性。
wTAM可从http://www.cuilab.cn/wtam/免费获取。
补充数据可在网上获取。
