蜡样芽孢杆菌生长和芽孢形成过程中二氢吡啶二羧酸合酶的调控
Regulation of dihydrodipicolinate synthase during growth and sporulation of Bacillus cereus.
作者信息
Hoganson D A, Stahly D P
出版信息
J Bacteriol. 1975 Dec;124(3):1344-50. doi: 10.1128/jb.124.3.1344-1350.1975.
Abstract
A four- to sixfold increase in specific activity of dihydrodipicolinic acid synthase was observed during sporulation of Bacillus cereus. The enzyme from cells harvested before and after the increase in specific activity appeared to be very similar as judged by pH optima, heat denaturation kinetics, apparent Michaelis constants, chromatography on diethylaminoethyl-cellulose and Sephadex G-200, and polyacrylamide gel electrophoresis. Studies with various combinations of amino acids and one of the enzyme substrates, pyruvate, failed to give evidence for control of the enzyme by activation, inhibition, repression, induction, or stabilization. Omission of calcium from the sporulation medium had no significant effect on the specific activity pattern of the enzyme as a function of age of culture.
摘要
在蜡样芽孢杆菌的孢子形成过程中，观察到二氢吡啶二羧酸合酶的比活性增加了四到六倍。从比活性增加之前和之后收获的细胞中提取的酶，根据最适pH值、热变性动力学、表观米氏常数、在二乙氨基乙基纤维素和葡聚糖G-200上的色谱分析以及聚丙烯酰胺凝胶电泳判断，似乎非常相似。用各种氨基酸组合和一种酶底物丙酮酸进行的研究，未能提供该酶通过激活、抑制、阻遏、诱导或稳定化进行调控的证据。从孢子形成培养基中省略钙，对该酶的比活性模式作为培养时间的函数没有显著影响。
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