Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Department of Dermatology, The Second People's Hospital of Hefei City, Hefei, China.
J Cosmet Dermatol. 2023 Apr;22(4):1361-1368. doi: 10.1111/jocd.15577. Epub 2023 Jan 26.
The aim of this study was to explore the anti-inflammatory and anti-lipid effects of lactoferrin on SZ95 human sebaceous gland cells and mouse model of acne.
SZ95 cells were co-cultured with different concentrations of lactoferrin, and cell viability was determined using the 2,5-diphenyl-2H-tetrazolium bromide method. Oil red O and Nile red staining were performed to determine the lipid content. The mRNA expression of genes related to lipid metabolism (sterol regulatory element-binding protein-1 [SREBP-1], fatty acid synthase [FAS], stearoyl-CoA desaturase-1 [SCD-1], fatty acid desaturase 2 [FADS2]) and inflammation (interleukin-8 [IL-8]) was determined by reverse transcription-polymerase chain reaction. An acne mouse model was established using injection of P. acnes on the backs of mice. The proliferation and apoptosis of sebaceous gland cells were examined by immunohistochemistry against proliferating cell nuclear antigen (PCNA) and TUNEL staining, respectively. Western blotting was used to detect FADS2 and CXCL15 protein expression.
Lactoferrin treatment at 10-500 μg/ml significantly decreased the lipid content, as revealed by the oil red O and Nile red staining. It also attenuated the increase of mRNA expression of SREBP-1, FAS, SCD-1, FADS2, and IL-8 in insulin-treated SZ95 cells. Moreover, lactoferrin treatment at the doses of 1-50 mg/mouse significantly reduced the inflammation and lipid production in the mouse model of acne. Also, the number of sebaceous gland cells was significantly reduced, and apoptosis was significantly increased by lactoferrin treatment in the mice. Mechanically, the levels of FADS2 and CXCL15 proteins in tissues were significantly decreased after lactoferrin treatment in the model mice.
Our results demonstrate the potential of lactoferrin against sebogenesis, sebaceous gland inflammation in acne.
本研究旨在探讨乳铁蛋白对 SZ95 人皮脂腺细胞和痤疮小鼠模型的抗炎和抗脂质作用。
将 SZ95 细胞与不同浓度的乳铁蛋白共培养，采用 2,5-二苯基-2H-四唑溴盐法测定细胞活力。用油红 O 和尼罗红染色法测定脂质含量。采用逆转录-聚合酶链反应测定与脂质代谢（固醇调节元件结合蛋白-1[SREBP-1]、脂肪酸合酶[FAS]、硬脂酰辅酶 A 去饱和酶-1[SCD-1]、脂肪酸去饱和酶 2[FADS2]）和炎症（白细胞介素-8[IL-8]）相关的基因的 mRNA 表达。通过在小鼠背部注射 P. acnes 建立痤疮小鼠模型。通过增殖细胞核抗原（PCNA）免疫组化和 TUNEL 染色分别检测皮脂腺细胞的增殖和凋亡。采用 Western blot 检测 FADS2 和 CXCL15 蛋白表达。
10-500μg/ml 的乳铁蛋白处理可显著减少油红 O 和尼罗红染色显示的脂质含量。它还可减轻胰岛素处理的 SZ95 细胞中 SREBP-1、FAS、SCD-1、FADS2 和 IL-8 的 mRNA 表达增加。此外，1-50mg/mouse 的乳铁蛋白处理可显著减轻痤疮小鼠模型的炎症和脂质生成。而且，乳铁蛋白处理可使小鼠的皮脂腺细胞数量明显减少，凋亡明显增加。在模型小鼠中，乳铁蛋白处理后组织中 FADS2 和 CXCL15 蛋白水平明显降低。
我们的研究结果表明乳铁蛋白具有抑制皮脂生成、痤疮皮脂腺炎症的潜力。
