Cheng Yahong, Sun Qingmiao, Gao Jiefang, Liu Qin, Tian Hui, Ding Hong, Qiao Jianjun, Chen Haijiang
College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, Hubei, 430023, P.R. China.
Department of Dermatology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310012, P.R. China.
Arch Dermatol Res. 2025 Feb 6;317(1):349. doi: 10.1007/s00403-025-03840-9.
Sebum composition may be more important than amount for acne lesions, and current research on skin surface lipids (SSLs) focuses on determining their relative content. The objective of this study was to analyze the changes in the absolute content of SSLs in acne patients and their relationship with skin barrier function. To evaluate skin barrier function, transepidermal water loss (TEWL), skin moisture, sebum content, skin elasticity, and whiteness were measured, while SSL changes were investigated using LC-MS/MS. The results indicated that adult acne patients have reduced skin barrier function, as demonstrated by changes in skin moisture, sebum content, skin flexibility, and whitening. Notably, AGlcSiE, Cer, CL, Co, LPC, PA, PC, PE, PI, SM, So, SQDG, and TG were considerably enhanced in acne patients' SSLs, whereas CerG1, DG, DGDG, MGDG, PG, and phSM were decreased. Furthermore, side chain analysis showed that the ratio of linoleic acid to linolenic acid in acne patients' skin surface lipids was higher than in healthy controls, and the caprylic acid/capric acid ratio was likewise greater. The correlation study of SSLs and skin barrier function demonstrated that increasing LPC and decreasing PG are associated with skin barrier function deterioration. In conclusion, acne patients have compromised skin barrier function and altered SSL absolute content, and certain SSL species identified in this study could serve as potential targets for research into acne pathogenesis.
对于痤疮皮损而言，皮脂成分可能比皮脂分泌量更为重要，目前关于皮肤表面脂质（SSLs）的研究主要集中在确定它们的相对含量。本研究的目的是分析痤疮患者皮肤表面脂质绝对含量的变化及其与皮肤屏障功能的关系。为评估皮肤屏障功能，测量了经表皮水分流失（TEWL）、皮肤水分、皮脂含量、皮肤弹性和白度，同时使用液相色谱-串联质谱法（LC-MS/MS）研究皮肤表面脂质的变化。结果表明，成年痤疮患者的皮肤屏障功能降低，这通过皮肤水分、皮脂含量、皮肤柔韧性和美白情况的变化得以体现。值得注意的是，痤疮患者皮肤表面脂质中的AGlcSiE、神经酰胺（Cer）、心磷脂（CL）、辅酶Q（Co）、溶血磷脂酰胆碱（LPC）、磷脂酸（PA）、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）、鞘磷脂（SM）、角鲨烯（So）、硫代异鼠李糖甘油二酯（SQDG）和甘油三酯（TG）显著增加，而神经酰胺G1（CerG1）、甘油二酯（DG）、二半乳糖甘油二酯（DGDG）、单半乳糖甘油二酯（MGDG）、磷脂酰甘油（PG）和磷酸鞘磷脂（phSM）则减少。此外，侧链分析表明，痤疮患者皮肤表面脂质中亚油酸与亚麻酸的比例高于健康对照，辛酸/癸酸比例同样更高。皮肤表面脂质与皮肤屏障功能的相关性研究表明，LPC增加和PG减少与皮肤屏障功能恶化有关。总之，痤疮患者的皮肤屏障功能受损，皮肤表面脂质绝对含量改变，本研究中鉴定出的某些皮肤表面脂质种类可作为痤疮发病机制研究的潜在靶点。
