Department of Biosystems Engineering, Seoul National University, Seoul 08826, Korea.
Convergence Major in Global Smart Farm, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
ACS Biomater Sci Eng. 2023 Feb 13;9(2):968-977. doi: 10.1021/acsbiomaterials.2c01367. Epub 2023 Jan 26.
Developing a scaffold for efficient and functional bone regeneration remains challenging. To accomplish this goal, a "scaffold-on-a-chip" device was developed as a platform to aid with the evaluation process. The device mimics a microenvironment experienced by a transplanted bone scaffold. The device contains a circular space at the center for scaffold insert and microfluidic channel that encloses the space. Such a design allows for monitoring of cell behavior at the blood-scaffold interphase. MC3T3-E1 cells were cultured with three different types of scaffold inserts to test its capability as an evaluation platform. Cellular behaviors, including migration, morphology, and osteogenesis with each scaffold, were analyzed through fluorescence images of live/dead assay and immunocytochemistry. Cellular behaviors, such as migration, morphology, and osteogenesis, were evaluated. The results revealed that our platform could effectively evaluate the osteoconductivity and osteoinductivity of scaffolds with various properties. In conclusion, our proposed platform is expected to replace current animal models as a highly relevant platform and can contribute to the fundamental study of bone regeneration.
开发高效且功能齐全的骨再生支架仍然具有挑战性。为了实现这一目标，开发了一种“芯片上的支架”设备作为辅助评估过程的平台。该设备模拟了移植骨支架所经历的微环境。该设备的中心有一个圆形空间用于支架插入，周围是微流道。这种设计允许监测血液-支架界面处的细胞行为。将 MC3T3-E1 细胞与三种不同类型的支架插入物一起培养，以测试其作为评估平台的能力。通过活/死检测和免疫细胞化学的荧光图像分析了每种支架的细胞迁移、形态和成骨行为。评估了细胞迁移、形态和成骨等行为。结果表明，我们的平台可以有效地评估具有不同特性的支架的骨传导性和骨诱导性。总之，我们提出的平台有望替代当前的动物模型，成为一个高度相关的平台，并为骨再生的基础研究做出贡献。
