Institute of Biomaterials and Bioengineering, Faculty of Natural Sciences and Technology, Riga Technical University, Pulka Street 3, Riga, LV-1007, Latvia.
Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga, LV-1007, Latvia.
Adv Healthc Mater. 2024 Nov;13(29):e2401307. doi: 10.1002/adhm.202401307. Epub 2024 Aug 23.
The replication of bone physiology under laboratory conditions is a prime target behind the development of in vitro bone models. The model should be robust enough to elicit an unbiased response when stimulated experimentally, giving reproducible outcomes. In vitro bone tissue generation majorly requires the availability of cellular components, the presence of factors promoting cellular proliferation and differentiation, efficient nutrient supply, and a supporting matrix for the cells to anchor - gaining predefined topology. Calcium phosphates (CaP) are difficult to ignore while considering the above requirements of a bone model. Therefore, the current review focuses on the role of CaP in developing an in vitro bone model addressing the prerequisites of bone tissue generation. Special emphasis is given to the physico-chemical properties of CaP that promote osteogenesis, angiogenesis and provide sufficient mechanical strength for load-bearing applications. Finally, the future course of action is discussed to ensure efficient utilization of CaP in the in vitro bone model development field.
在实验室条件下复制骨骼生理学是体外骨模型发展的主要目标。该模型应该足够强大，以便在实验刺激时产生无偏倚的反应，从而产生可重复的结果。体外骨组织的生成主要需要细胞成分的可用性、促进细胞增殖和分化的因子的存在、有效的营养供应以及细胞附着的支撑基质-获得预定义的拓扑结构。在考虑骨模型的上述要求时，钙磷（CaP）是不容忽视的。因此，本综述重点介绍了 CaP 在开发体外骨模型中的作用，该模型满足骨组织生成的前提条件。特别强调了促进成骨、血管生成和为承重应用提供足够机械强度的 CaP 的物理化学性质。最后，讨论了未来的行动方案，以确保 CaP 在体外骨模型开发领域的有效利用。
