Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore, 117575, Singapore.
Sci Rep. 2024 Sep 10;14(1):21097. doi: 10.1038/s41598-024-72073-z.
Polymer composite scaffolds hold promise in bone tissue engineering due to their biocompatibility, mechanical properties, and reproducibility. Among these materials, polylactic acid (PLA), a biodegradable plastics has gained attention for its processability characteristics. However, a deeper understanding of how PLA scaffold surface properties influence cell behavior is enssential for advancing its applications. In this study, 3D-printed PLA scaffolds containing hydroxyapatite (HA) were analyzed using atomic force microscopy and nanomechanical mapping. The addition of HA significantly increased key surface properties compared to unmodified PLA scaffols. Notably, the HA-modified scaffold demonstrated Gaussian distribution of stiffness and adhesive forces, in contrast to the bimodal properties observed in the unmodified PLA scaffolds. Human adipose-derived mesenchymal stem cell (hADMSC) seeded on the 3D-printed PLA scaffolds blended with 10% HA (P10) exhibited strong attachment. After four weeks, osteogenic differentiation of hADMSCs was detected, with calcium deposition reaching 6.76% ± 0.12. These results suggest that specific ranges of stiffness and adhesive forces of the composite scaffold can support cell attachement, and mineralization. The study highlights that tailoring suface properties of composite scaffolds is crucial for modulating cellular interactions, thus advancing the development of effective bone replacement materials.
基于其生物相容性、机械性能和可重复性，聚合物复合材料支架在骨组织工程中具有广阔的应用前景。在这些材料中，聚乳酸（PLA）作为一种可生物降解塑料，由于其加工性能而受到关注。然而，深入了解 PLA 支架表面特性如何影响细胞行为对于推进其应用至关重要。在这项研究中，使用原子力显微镜和纳米力学映射对含有羟基磷灰石（HA）的 3D 打印 PLA 支架进行了分析。与未改性的 PLA 支架相比，添加 HA 显著提高了关键表面特性。值得注意的是，与未改性的 PLA 支架观察到的双峰特性相比，HA 改性支架表现出弹性和粘附力的高斯分布。在 3D 打印 PLA 支架上接种了 10%HA（P10）的人脂肪间充质干细胞（hADMSC）表现出很强的附着性。四周后，检测到 hADMSCs 的成骨分化，钙沉积达到 6.76%±0.12。这些结果表明，复合支架的特定弹性和粘附力范围可以支持细胞附着和矿化。该研究强调，调整复合支架的表面特性对于调节细胞相互作用至关重要，从而推进有效的骨替代材料的发展。
