Department of Chemistry, Texas A&M University, College Station, Texas77843, United States.
Department of Chemistry, Washington State University, Pullman, Washington99164, United States.
Anal Chem. 2023 Feb 7;95(5):3062-3068. doi: 10.1021/acs.analchem.2c05221. Epub 2023 Jan 26.
Digital mass filters are advantageous for the analysis of large molecules due to the ability to perform ion isolation of high-/ ions without the generation of very high radio frequency (RF) and DC voltages. Experimentally determined Mathieu stability diagrams of stability zone 1,1 for capacitively coupled digital waveforms show a voltage offset between the quadrupole rod pairs is introduced by the capacitors which is dependent on the voltage magnitude of the waveform and the duty cycle. This changes the ion's value from = 0 to < 0. These effects are illustrated for isolation for single-charge states for various protein complexes up to 800 kDa (GroEL) for stability zone 1,1. Isolation resolving power (/Δ) of approximately 280 was achieved for an ion of / 12,315 ( = 65+ for 800.5 kDa GroEL D398A), which corresponds to an / window of 44.
数字质量滤波器由于能够在不产生非常高的射频 (RF) 和直流电压的情况下对高-/离子进行离子隔离，因此在分析大分子方面具有优势。实验确定的电容耦合数字波形稳定区 1,1 的 Mathieu 稳定性图表明，在四极杆对之间引入了电压偏移，该电压偏移取决于波形的电压幅度和占空比。这将离子的 值从 = 0 变为 < 0。对于各种蛋白质复合物（高达 800 kDa（GroEL）的单电荷状态的隔离，这些效应得到了说明。对于 / 12,315（= 65+对于 800.5 kDa GroEL D398A）的离子，实现了约 280 的隔离分辨率 (/Δ)，这对应于 / 窗口为 44。
