Stauffer J L, Potts D E, Sahn S A
Acta Cytol. 1978 Nov-Dec;22(6):570-4.
The cellular content of the pleural space of five normal rabbits was studied by three cytopreparative techniques using different methods of collection (aspiration, irrigation) and fixation (95% ethyl alcohol, 50% alcohol: 1% polyethylene glycol). The method using aspiration, 95% ethyl alcohol fixation, and the Papanicolaou stain was simple and provided the best cytologic detail. With this method 70.1% of pleural fluid cells were monocytic, 7.5% were monocytic with morphologic features typical of macrophages, 10.5% were lymphocytes, 8.9% were mesothelial cells and 1.9% were polymorphonuclear leukocytes. Other methods were technically more difficult and provided less cytologic detail. The cellular content of the normal rabbit pleural space is described, providing baseline information for the study of experimental pleural disease.
采用三种细胞制备技术，运用不同的采集（抽吸、灌洗）和固定方法（95%乙醇、50%乙醇：1%聚乙二醇），对5只正常兔胸膜腔的细胞成分进行了研究。采用抽吸、95%乙醇固定及巴氏染色的方法简单，能提供最佳的细胞学细节。用这种方法，70.1%的胸水细胞为单核细胞，7.5%为具有巨噬细胞典型形态特征的单核细胞，10.5%为淋巴细胞，8.9%为间皮细胞，1.9%为多形核白细胞。其他方法在技术上更困难，提供的细胞学细节更少。描述了正常兔胸膜腔的细胞成分，为实验性胸膜疾病的研究提供了基线信息。
