Antony V B, Sahn S A, Antony A C, Repine J E
J Clin Invest. 1985 Oct;76(4):1514-21. doi: 10.1172/JCI112131.
Neutrophils are often seen first at sites of granulomatous inflammation but their contribution to monocyte recruitment and granuloma formation is unknown. We tested the hypothesis that neutrophils release chemotaxins which attract monocytes. We found that rapid accumulations of fluid and influxes of neutrophils followed by monocytes occurred in bacillus Calmette--Guérin (BCG)-sensitized rabbits given BCG intrapleurally but did not occur in nitrogen mustard-treated (neutropenic) BCG-sensitized rabbits given BCG intrapleurally--unless the rabbits were also given intrapleural injections of neutrophils. We also found monocyte chemotaxins in pleural spaces of control and neutrophil-reconstituted neutropenic but not in neutropenic rabbits given BCG intrapleurally. Moreover, pleural fluid monocyte chemotaxins had molecular weights (12,000-15,000 and 1,000) that were similar to molecular weights of monocyte chemotaxins present in supernatants from mixtures of neutrophils and BCG in vitro. In addition, intrapleural injection of neutrophils and BCG or supernatants from in vitro mixtures of neutrophils and BCG (but not neutrophils or BCG alone) increased the numbers of monocytes and 3H cell pellet activity in pleural fluids from untreated neutropenic rabbits or neutropenic rabbits previously injected intravenously with 3[H]methyl thymidine-labeled monocytes. Furthermore, fewer BCG were recovered from pleural fluids of BCG-sensitized control compared to neutropenic rabbits given BCG, and at autopsy 10 d after instillation of BCG, control but not neutropenic rabbits had well-defined granulomas without adhesions on their pleural surfaces. Our results suggest that BCG stimulates neutrophils to release chemotaxins that recruit monocytes, and that these responses might contribute to granuloma formation in tuberculous pleurisy.
在肉芽肿性炎症部位常最先见到中性粒细胞，但它们对单核细胞募集和肉芽肿形成的作用尚不清楚。我们检验了中性粒细胞释放吸引单核细胞的趋化因子这一假说。我们发现，对卡介苗（BCG）致敏的家兔经胸腔内注射BCG后，会迅速出现液体蓄积和中性粒细胞流入，随后是单核细胞流入，但在经氮芥处理（中性粒细胞减少）的BCG致敏家兔经胸腔内注射BCG时则不会出现这种情况——除非这些家兔也经胸腔内注射中性粒细胞。我们还发现，在对照家兔以及中性粒细胞重建的中性粒细胞减少家兔的胸腔积液中有单核细胞趋化因子，而在经胸腔内注射BCG的中性粒细胞减少家兔的胸腔积液中则没有。此外，胸腔积液中的单核细胞趋化因子的分子量（12,000 - 15,000和1,000）与体外中性粒细胞和BCG混合物上清液中存在的单核细胞趋化因子的分子量相似。另外，对未经处理的中性粒细胞减少家兔或先前经静脉注射3[H]甲基胸腺嘧啶核苷标记单核细胞的中性粒细胞减少家兔，经胸腔内注射中性粒细胞和BCG或中性粒细胞与BCG体外混合物的上清液（但不是单独的中性粒细胞或BCG）可增加胸腔积液中单核细胞的数量和3H细胞沉淀活性。此外，与经BCG处理的中性粒细胞减少家兔相比，BCG致敏对照家兔胸腔积液中回收的BCG较少，并且在滴注BCG后10天尸检时，对照家兔而非中性粒细胞减少家兔有界限清楚的肉芽肿，胸膜表面无粘连。我们的结果提示，BCG刺激中性粒细胞释放趋化因子以募集单核细胞，并且这些反应可能有助于结核性胸膜炎中肉芽肿的形成。
