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对不同发育阶段白纹伊蚊（双翅目：蚊科）的全转录组进行分析，揭示了几丁质相关非编码 RNA 和竞争内源性 RNA 网络。

Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks.

机构信息

School of Clinical and Basic Medical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, 250117, China.

Shandong Institute of Parasitic Diseases, Shandong First Medical University (Shandong Academy of Medical Sciences), Jining, 272033, China.

出版信息

Parasit Vectors. 2023 Jan 26;16(1):33. doi: 10.1186/s13071-022-05648-2.

DOI:10.1186/s13071-022-05648-2
PMID:36703236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9878986/
Abstract

BACKGROUND

The Asian tiger mosquito, Aedes albopictus, is one of the most invasive species and a vector of numerous arboviruses. The deleterious effects of long-term and inappropriate use of chemical pesticides have stimulated the exploration of new, environmentally friendly control strategies. Non-coding RNAs (ncRNAs) have been proven to participate in almost all biological processes of insects.

METHODS

In this study, circular RNAs (circRNAs) and microRNAs (miRNAs) covering five developmental stages [egg, early larvae, late larvae, pupae, adult (female and male)] of A. albopictus were obtained using whole-transcriptome sequencing technology. Combined with long non-coding RNAs (lncRNAs) from previous research, circRNA/lncRNA‒miRNA‒mitochondrial RNA (mRNA) networks were constructed.

RESULTS

A total of 1434 circRNAs and 208 miRNAs were identified. More differentially expressed circRNAs (DE circRNAs) and miRNAs (DE miRNAs) were found in the egg versus early larvae comparison group. Functional enrichment analysis demonstrated that most of the circRNA/lncRNA‒miRNA‒mRNA networks were involved in chitin metabolism. Hub genes of each circRNA/lncRNA‒miRNA‒mRNA network were screened out, which can be used as novel targets to disturb the molting process of A. albopictus.

CONCLUSIONS

Regulatory relationships obtained from competing endogenous RNA (ceRNA) networks provide more information to manipulate the metamorphosis process and are helpful for developing effective and sustainable methods to control mosquitoes.

摘要

背景

亚洲虎蚊（Aedes albopictus）是最具侵袭性的物种之一，也是许多虫媒病毒的传播媒介。长期、不恰当地使用化学农药所带来的有害影响，刺激了人们对新的、环保的控制策略的探索。非编码 RNA（ncRNA）已被证明参与了昆虫几乎所有的生物学过程。

方法

在本研究中，我们使用全转录组测序技术获得了 A. albopictus 五个发育阶段（卵、早期幼虫、晚期幼虫、蛹、成虫（雌、雄））的环状 RNA（circRNA）和 microRNA（miRNA）。结合之前研究中的长非编码 RNA（lncRNA），构建了 circRNA/lncRNA-miRNA-线粒体 RNA（mRNA）网络。

结果

共鉴定出 1434 个 circRNA 和 208 个 miRNA。在卵与早期幼虫的比较组中发现了更多差异表达的 circRNA（DE circRNA）和 miRNA（DE miRNA）。功能富集分析表明，大多数 circRNA/lncRNA-miRNA-mRNA 网络都参与了几丁质代谢。筛选出每个 circRNA/lncRNA-miRNA-mRNA 网络的枢纽基因，可作为扰乱 A. albopictus 蜕皮过程的新靶标。

结论

ceRNA 网络中的调控关系提供了更多信息来操纵变态过程，有助于开发有效和可持续的控制蚊子的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/5b28e43692c4/13071_2022_5648_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/fa8bf76674f5/13071_2022_5648_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/d5a40eba1bfa/13071_2022_5648_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/119c869a4a8a/13071_2022_5648_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/91334b8d2733/13071_2022_5648_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/30b0bb910a6b/13071_2022_5648_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/5b28e43692c4/13071_2022_5648_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/fa8bf76674f5/13071_2022_5648_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/d5a40eba1bfa/13071_2022_5648_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/119c869a4a8a/13071_2022_5648_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/91334b8d2733/13071_2022_5648_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/30b0bb910a6b/13071_2022_5648_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f201/9878986/5b28e43692c4/13071_2022_5648_Fig6_HTML.jpg

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