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摩洛哥草莓技术管理调查及其采后微生物负荷评估

Survey on technical management of strawberries in Morocco and evaluation of their post-harvest microbial load.

作者信息

El-Araby Abir, Azzouzi Amal, Ayam Iman Msegued, Samouh Khaoula Filali, Errachidi Faouzi

机构信息

Functional Ecology and Environment Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, Fez, Morocco.

Department of Biology, Immunology and Biodiversity Laboratory, Faculty of Sciences Ain Chock, University Hassan II, Casablanca, Morocco.

出版信息

Front Microbiol. 2023 Jan 10;13:1115340. doi: 10.3389/fmicb.2022.1115340. eCollection 2022.

Abstract

The climatic conditions of the growing regions influence the fruit's microbiological quality and their tolerance to post-harvest pathogens. The present work aims to identify the prevalence of bacterial and fungal strains of strawberries (Fragaria ananassa) in the Gharb and Loukkos regions of Morocco. Thus, to establish a correlation between the microbial load and the climatic conditions of the two targeted regions. The bacteriological analyses were studied according to the International Organization for Standardization (ISO) methodologies. Regarding the mycological study, fungal species determination was performed using identification keys. Yeast species determination was done using genus analysis, assimilation, and fermentation tests. Emberger bioclimatic quotients (Q) were calculated for the Gharb and Loukkos regions and bioclimatic stages were determined. spp. was not detected in the studied samples. However, and were isolated from the Gharb samples. Sulfite-reducing clostridia spores were found in two Gharb samples versus one Loukkos sample. Coagulase-positive staphylococci were negative in all samples analyzed. Loukkos and Gharb regions were contaminated by with percentages of occurrence of 5.2 and 7.8%, respectively. The fungi found in strawberries from both regions were , , spp., spp., spp., and with a significant predominance in the Loukkos samples. Indeed, a marked presence is noted for and in strawberries from Loukkos. Gharb is located on the semi-arid stage, while Loukkos is located on the sub-humid stage. Climatic conditions have a strong influence on plant microbial load, which explains the prevalence of bacteria in strawberries from Gharb and the prevalence of fungi in strawberries from Loukkos.

摘要

种植地区的气候条件会影响水果的微生物质量及其对采后病原体的耐受性。本研究旨在确定摩洛哥加卜和卢科斯地区草莓(Fragaria ananassa)的细菌和真菌菌株的流行情况。因此,要在这两个目标地区的微生物负荷与气候条件之间建立相关性。细菌学分析是根据国际标准化组织(ISO)的方法进行研究的。关于真菌学研究,使用鉴定检索表来确定真菌种类。酵母种类的确定是通过属分析、同化和发酵试验来完成的。计算了加卜和卢科斯地区的恩贝格尔生物气候商数(Q)并确定了生物气候阶段。在所研究的样本中未检测到 spp.。然而,从加卜地区的样本中分离出了 和 。在两个加卜地区的样本中发现了亚硫酸盐还原梭菌孢子,而在卢科斯地区的样本中仅发现一个。在所有分析的样本中,凝固酶阳性葡萄球菌均为阴性。卢科斯和加卜地区均受到 污染,其出现百分比分别为5.2%和7.8%。在这两个地区的草莓中发现的真菌有 、 、 spp.、 spp.、 spp.和 ,在卢科斯地区的样本中占显著优势。事实上,在卢科斯地区的草莓中明显存在 和 。加卜地区处于半干旱阶段,而卢科斯地区处于亚湿润阶段。气候条件对植物的微生物负荷有很大影响,这解释了加卜地区草莓中细菌的流行以及卢科斯地区草莓中真菌的流行。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/9871894/f9d813d95c92/fmicb-13-1115340-g001.jpg

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