Department of Pathology, B.P. Koirala Institute of Health Sciences, Dharan, Sunsari, Nepal.
Department of Radiodiagnosis and Imaging, B.P. Koirala Institute of Health Sciences, Dharan, Sunsari, Nepal.
JNMA J Nepal Med Assoc. 2022 Dec 1;60(256):1037-1040. doi: 10.31729/jnma.7909.
Micronucleus is used as a biomarker of chromosomal instability, which is one of the hallmarks of neoplastic transformation. As micronuclei score increases with malignancy, it can be an effective and inexpensive adjunct to breast fine needle aspiration cytology, in diagnosing breast lumps, especially detecting grey lesions between benign and malignant tumours. The aim of this study is to find out the mean micronuclei score in fine-needle aspiration cytology of patients with malignant breast lumps in the Department of Pathology in a tertiary care centre.
A descriptive cross-sectional study was conducted among patients with malignant breast lumps in the Department of Pathology in a tertiary care centre between 1 May 2020 to 31 May 2021 after receiving ethical approval from the Institutional Review Committee (Reference number: IRC/2139/021). The fine-needle aspiration cytology of breast lumps was diagnosed as per National Health services breast screening program guidelines. The mean micronuclei score was calculated. Convenience sampling was done and data were collected from the hospital records in the Department. Point estimate and 95% Confidence Interval were calculated.
Among 20 malignant breast aspirates, the mean micronuclei score was found to be 8.30±3.75 (3-19, 95% Confidence Interval).
The mean micronuclei score in fine-needle aspiration cytology of malignant breast lumps was found to be similar when compared to similar studies conducted in similar settings.
biomarker; breast neoplasm; chromosomal instability; fine-needle aspiration.
微核可作为染色体不稳定性的生物标志物，而后者是肿瘤转化的标志之一。由于微核评分随着恶性程度的增加而增加，因此它可以作为乳腺细针抽吸细胞学诊断的有效且廉价的辅助手段，特别适用于诊断乳腺肿块，尤其是检测良性和恶性肿瘤之间的灰色病变。本研究旨在探讨在三级保健中心的病理学系中，恶性乳腺肿块的细针抽吸细胞学检查中的平均微核评分。
在获得机构审查委员会的伦理批准后（参考编号：IRC / 2139 / 021），在 2020 年 5 月 1 日至 2021 年 5 月 31 日期间，在三级保健中心的病理学系中对患有恶性乳腺肿块的患者进行了描述性横断面研究。根据国家卫生服务乳腺筛查计划指南诊断乳腺肿块的细针抽吸细胞学检查。计算平均微核评分。采用便利抽样法，从医院记录中收集数据。计算点估计值和 95%置信区间。
在 20 例恶性乳腺抽吸物中，平均微核评分发现为 8.30±3.75（3-19，95%置信区间）。
与在类似环境中进行的类似研究相比，恶性乳腺肿块的细针抽吸细胞学检查中的平均微核评分相似。
生物标志物；乳腺肿瘤；染色体不稳定性；细针抽吸。
