Dey Pranab, Samanta Swapan, Susheilia Shaily
Department of Cytology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Diagn Cytopathol. 2012 Aug;40(8):664-6. doi: 10.1002/dc.21589. Epub 2010 Dec 31.
The aim of this study is to compare micronucleus assay in buccal smear of breast carcinoma patients versus normal benign cases as control group. In this prospective study, we selected a total 32 patients of carcinoma of breast and 49 patients of benign breast lesions diagnosed in fine needle aspiration cytology (FNAC). Acridine orange stain was done on buccal smears of these cases and micronucleus (MN) scoring was performed in 40× 0bjective in a fluorescent microscope. The MN score was expressed as positivity per 1,000 cells. The MN scoring in buccal smear was compared in malignant and benign breast cases. In fluorescence microscope, the micronucleus was detected as round orange shaped small intracytoplasmic structure around the nucleus. The mean MN scores in buccal smears of benign and carcinoma cases were 0.5014 ± 0.45768 and 2.1938 ± 1.08656 cases respectively. Independent sample Student's t test showed significantly high MN score in buccal smear of the cancer patients (P < 0.001). Micronucleated cells are significantly increased in buccal cells of the breast carcinoma cases. The increased number of MN in buccal smears raises the possibility that the genetic damage in breast cancer patients is generalized. In future, MN scoring could be used as biomonitoring of DNA damage and in early detection of high risk cases of carcinoma of breast.
本研究的目的是比较乳腺癌患者口腔涂片微核试验与作为对照组的正常良性病例。在这项前瞻性研究中，我们共选取了32例经细针穿刺细胞学检查（FNAC）确诊的乳腺癌患者和49例乳腺良性病变患者。对这些病例的口腔涂片进行吖啶橙染色，并在荧光显微镜下用40倍物镜进行微核（MN）评分。MN评分以每1000个细胞中的阳性率表示。比较了恶性和良性乳腺病例口腔涂片的MN评分。在荧光显微镜下，微核被检测为细胞核周围圆形橙色的小细胞质内结构。良性和癌性病例口腔涂片的平均MN评分分别为0.5014±0.45768和2.1938±1.08656。独立样本t检验显示癌症患者口腔涂片的MN评分显著较高（P<0.001）。乳腺癌病例的口腔细胞中微核化细胞显著增加。口腔涂片中MN数量的增加增加了乳腺癌患者基因损伤具有普遍性的可能性。未来，MN评分可用于DNA损伤的生物监测以及乳腺癌高危病例的早期检测。
