Yazici-Mutlu Çiğdem, Keskin-Aktan Arzu, Akbulut Kazime G
Department of Physiotherapy and Rehabilitation, Yeditepe University, Health Science Faculty, Istanbul, Turkey.
Gen Physiol Biophys. 2023 Jan;42(1):49-57. doi: 10.4149/gpb_2022047.
The study investigated the effect of exogenous melatonin and (or) curcumin treatment on the cerebral cortex of adult rats. In this context, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), superoxide dismutase (SOD), nuclear factor E2-related factor 2 (Nrf2) and SIRT2 protein expression were examined. A total of 30 Wistar albino rats involved in the study were randomly divided into five groups. Over 30 days, the control groups received phosphate-buffered saline or dimethyl sulfoxide injections, and the treatment groups received melatonin, curcumin, or a combination of melatonin and curcumin injections. In the cerebral cortex homogenates, the MDA, GSH, and sum of NO were respectively determined by the thiobarbituric acid, modified Ellman and Griess test methods. The SOD and Nrf2 levels were examined using the ELISA method and SIRT2 protein expression using the Western blot technique. The study found that both melatonin and curcumin treatments significantly reduced lipid peroxidation and SIRT2 protein expression levels (p < 0.05) and increased the Nrf2 level in the cytoplasm (p < 0.05). The study revealed that curcumin and melatonin treatments reduced MDA and SIRT2 protein expression level and increased intracellular Nrf2, GSH, and SOD in the cortex tissue. We also found that the combined melatonin and curcumin treatment produced no synergistic effect.
该研究调查了外源性褪黑素和（或）姜黄素处理对成年大鼠大脑皮层的影响。在此背景下，检测了丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、核因子E2相关因子2（Nrf2）和SIRT2蛋白表达。参与该研究的30只Wistar白化大鼠被随机分为五组。在30天的时间里，对照组接受磷酸盐缓冲盐水或二甲基亚砜注射，治疗组接受褪黑素、姜黄素或褪黑素与姜黄素联合注射。在大脑皮层匀浆中，分别采用硫代巴比妥酸法、改良Ellman法和Griess法测定MDA、GSH和NO总量。采用ELISA法检测SOD和Nrf2水平，采用蛋白质印迹技术检测SIRT2蛋白表达。研究发现，褪黑素和姜黄素处理均显著降低脂质过氧化和SIRT2蛋白表达水平（p<0.05），并增加细胞质中Nrf2水平（p<0.05）。该研究表明，姜黄素和褪黑素处理降低了皮层组织中MDA和SIRT2蛋白表达水平，并增加了细胞内Nrf2、GSH和SOD。我们还发现，褪黑素与姜黄素联合处理未产生协同效应。
