STV-Na 通过 TLR4/NF-κB 通路减轻脂多糖诱导的小鼠肺损伤。
STV-Na attenuates lipopolysaccharide-induced lung injury in mice via the TLR4/NF-kB pathway.
机构信息
Department of Respiratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Plastic Surgery, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang Medical University, Xinjiang, Urumqi, China.
出版信息
Immun Inflamm Dis. 2023 Jan;11(1):e770. doi: 10.1002/iid3.770.
BACKGROUND
Acute lung injury (ALI) is a potentially fatal disorder that is largely caused by inflammation. Sodium isostevanol (STV-Na) is a terpenoid produced from stevioside, which possesses anti-inflammatory and antioxidative stress characteristics. nevertheless, it is still unclear how STV-Na affects ALI. Therefore, we investigated the possible STV-Na therapeutic impacts on lipopolysaccharide (LPS)-induced (ALI).
METHODS
We employed hematoxylin-eosin staining to observe the impact of STV-Na on lung histopathological alterations and used kits to detect the oxidative stress status of lung tissues, such as superoxide dismutase, malondialdehyde, and glutathione. The reactive oxygen species and myeloperoxidase expression in the tissues of lung was assessed by immunofluorescence and immunohistochemistry. Additionally, we detected the impact of STV-Na on inflammatory cell infiltration in lung tissue using Wright-Giemsa staining solution and immunohistochemistry, which was found to reduce inflammation in lung tissue by enzyme-linked immunosorbent assay. Finally, using WB, we examined the impact of STV-Na on the TLR4/NF-kB pathway.
RESULTS
We observed that STV-Na attenuated lung histopathological alterations in LPS-induced lung damage in mice, reduced infiltration of inflammatory cell and oxidative stress in the tissue of lung, and via the TLR4/NF-kB pathway, there is a reduction in the inflammatory responses in mouse lung tissue.
CONCLUSIONS
These outcomes indicate that the response of inflammatory cells to LPS-induced ALI in mice was attenuated by STV-Na.
背景
急性肺损伤（ALI）是一种潜在致命的疾病，主要由炎症引起。钠异甜醇（STV-Na）是从甜菊糖衍生而来的萜烯，具有抗炎和抗氧化应激特性。然而，STV-Na 如何影响 ALI 仍不清楚。因此，我们研究了 STV-Na 对脂多糖（LPS）诱导的（ALI）的可能治疗作用。
方法
我们采用苏木精-伊红染色观察 STV-Na 对肺组织病理改变的影响，采用试剂盒检测肺组织氧化应激状态，如超氧化物歧化酶、丙二醛和谷胱甘肽。用免疫荧光和免疫组化检测组织中活性氧和髓过氧化物酶的表达。此外，我们用 Wright-Giemsa 染色液和免疫组化检测 STV-Na 对肺组织中炎症细胞浸润的影响，酶联免疫吸附试验发现 STV-Na 可减轻肺组织炎症。最后，用 WB 检测 STV-Na 对 TLR4/NF-kB 通路的影响。
结果
我们观察到 STV-Na 减轻了 LPS 诱导的肺损伤小鼠的肺组织病理改变，减少了肺组织中炎症细胞的浸润和氧化应激，通过 TLR4/NF-kB 通路，减轻了小鼠肺组织中的炎症反应。
结论
这些结果表明，STV-Na 减轻了炎症细胞对 LPS 诱导的 ALI 的反应。
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