Graduate Institute of Health Industry Technology, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Taoyuan City 33303, Taiwan.
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan City 33303, Taiwan.
Mediators Inflamm. 2021 Sep 7;2021:4544294. doi: 10.1155/2021/4544294. eCollection 2021.
Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice.
番茄碱是从绿番茄中分离出来的，可以改善细胞的炎症和氧化应激，并已被证明可以改善哮喘小鼠模型中的气道炎症。在这里，我们研究了番茄碱是否可以改善小鼠的急性肺损伤。通过腹腔注射连续 7 天给予小鼠番茄碱，然后通过气管内滴注脂多糖（LPS）诱导肺损伤。番茄碱降低了支气管肺泡灌洗液（BALF）中的炎性细胞因子表达，减轻了 BALF 和肺组织中的中性粒细胞浸润，增加了超氧化物歧化酶活性和谷胱甘肽水平，并减轻了肺组织中髓过氧化物酶的表达。番茄碱还降低了炎症肺部的炎性细胞因子和趋化因子基因表达，并减弱了丝裂原活化蛋白激酶和核因子 κB 的磷酸化。此外，番茄碱增强了血红素加氧酶-1 的产生，减少了 LPS 刺激的肺上皮细胞中炎性细胞因子和趋化因子的分泌，并减轻了 THP-1 单核细胞的黏附。我们的研究结果表明，番茄碱可减轻氧化应激和炎症，改善小鼠的急性肺损伤。
