Takizawa Naoki
Laboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
Methods Mol Biol. 2023;2586:217-231. doi: 10.1007/978-1-0716-2768-6_13.
RNA functions are closely linked with their structures. Therefore, elucidating the secondary structure of RNAs provides crucial information regarding their function. The chemical modification or RNase-mediated digestion of single-stranded RNA has been utilized to experimentally reveal RNA secondary structures. Owing to advances in high-throughput sequencing technology and chemical analysis, RNA structural analyses that enable structural profiling at the transcriptomic scale in living cells have been developed. Here, we provide an overview of the high-throughput RNA structural (HTS) analyses and describe the computational processing steps of recent HTS analysis pipelines: PROBer, BUMHMM, and reactIDR.
RNA的功能与其结构密切相关。因此，阐明RNA的二级结构可为其功能提供关键信息。单链RNA的化学修饰或核糖核酸酶介导的消化已被用于通过实验揭示RNA二级结构。由于高通量测序技术和化学分析的进步，已开发出能够在活细胞转录组规模上进行结构分析的RNA结构分析方法。在这里，我们概述了高通量RNA结构（HTS）分析，并描述了最近的HTS分析流程（PROBer、BUMHMM和reactIDR）的计算处理步骤。
