RNA Structure Determination by High-Throughput Structural Analysis.

RNA functions are closely linked with their structures. Therefore, elucidating the secondary structure of RNAs provides crucial information regarding their function. The chemical modification or RNase-mediated digestion of single-stranded RNA has been utilized to experimentally reveal RNA secondary structures. Owing to advances in high-throughput sequencing technology and chemical analysis, RNA structural analyses that enable structural profiling at the transcriptomic scale in living cells have been developed. Here, we provide an overview of the high-throughput RNA structural (HTS) analyses and describe the computational processing steps of recent HTS analysis pipelines: PROBer, BUMHMM, and reactIDR.