用于电化学检测系统性硬化症中抑瘤素M受体和单克隆自身抗体的纳米免疫传感器

Nanoimmunosensor for the electrochemical detection of oncostatin M receptor and monoclonal autoantibodies in systemic sclerosis.

作者信息

Avelino Karen Y P S, Silva-Junior Alberto G, Pitta Maira G R, Errachid Abdelhamid, Oliveira Maria D L, Andrade César A S

机构信息

Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.

Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.

出版信息

Talanta. 2023 May 1;256:124285. doi: 10.1016/j.talanta.2023.124285. Epub 2023 Jan 21.

DOI:10.1016/j.talanta.2023.124285

PMID:36706502

Abstract

Systemic sclerosis (SSc) is a chronic, autoimmune disease that primarily affects connective tissue. SSc can be classified into limited cutaneous (lSSc) and diffuse cutaneous (dSSc). Oncostatin M receptor (sOSMR) is an important inflammatory biomarker expressed in the serum of patients with autoimmune diseases. A nanoengineered immunosensor surface was developed. The biosensor was composed of a conductive layer of polypyrrole, electrodeposited gold nanoparticles, and sOSMR protein for anti-human OSMR monoclonal antibody biorecognition. The electrochemical response evaluated by cyclic voltammetry and electrochemical impedance spectroscopy indicated the detection of the target analyte present in clinical samples from lSSc and dSSc patients. The voltammetric anodic shift for lSSc specimens was 82.7% ± 0.9-93.6% ± 3.2, and dSSc specimens was 118.7 ± 2.6 to 379.6 ± 2.6, revealing a differential diagnostic character for SSc subtypes. The sensor platform was adapted for identifying sOSMR, using anti-OSMR antibodies as bioreceptors. With a linear response range estimated from 0.005 to 500 pg mL and a limit of detection of 0.42 pg mL, the sensing strategy demonstrated high sensitivity in identifying the human OSMR protein in clinical samples. The proposed biosensor is a promising and innovative tool for SSc-related biomarker research.

摘要

系统性硬化症（SSc）是一种主要影响结缔组织的慢性自身免疫性疾病。SSc可分为局限性皮肤型（lSSc）和弥漫性皮肤型（dSSc）。制瘤素M受体（sOSMR）是一种在自身免疫性疾病患者血清中表达的重要炎症生物标志物。开发了一种纳米工程免疫传感器表面。该生物传感器由聚吡咯导电层、电沉积金纳米颗粒和用于抗人OSMR单克隆抗体生物识别的sOSMR蛋白组成。通过循环伏安法和电化学阻抗谱评估的电化学响应表明，可检测lSSc和dSSc患者临床样本中的目标分析物。lSSc样本的伏安阳极位移为82.7%±0.9 - 93.6%±3.2，dSSc样本为118.7±2.6至379.6±2.6，揭示了SSc亚型的鉴别诊断特征。该传感器平台采用抗OSMR抗体作为生物受体来识别sOSMR。该传感策略的线性响应范围估计为0.005至500 pg/mL，检测限为0.42 pg/mL，在识别临床样本中的人OSMR蛋白方面表现出高灵敏度。所提出的生物传感器是用于SSc相关生物标志物研究的一种有前景的创新工具。