• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于聚吡咯的平台在未稀释血清中进行电化学 ELISA 蛋白质生物传感

Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platform.

机构信息

Department of Electronic & Electrical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, UK.

Centre for Biosensors, Bioelectronics and Biodevices (C3Bio), University of Bath, Claverton Down, Bath BA2 7AY, UK.

出版信息

Sensors (Basel). 2020 May 18;20(10):2857. doi: 10.3390/s20102857.

DOI:10.3390/s20102857
PMID:32443483
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7287672/
Abstract

An electrochemical enzyme-linked immunosorbent assay (ELISA) biosensor platform using electrochemically prepared ~11 nm thick carboxylic functionalized popypyrrole film has been developed for bio-analyte measurement in undiluted serum. Carboxyl polypyrrole (PPy-COOH) film using 3-carboxy-pyrrol monomer onto comb-shaped gold electrode microarray (Au) was prepared via cyclic voltammetry (CV). The prepared Au/PPy-COOH was then utilized for electrochemical ELISA platform development by immobilizing analyte-specific antibodies. Tumor necrosis factor-alpha (TNF-α) was selected as a model analyte and detected in undiluted serum. For enhanced performance, the use of a polymeric alkaline phosphatase tag was investigated for the electrochemical ELISA. The developed platform was characterized at each step of fabrication using CV, electrochemical impedance spectroscopy and atomic force microscopy. The bioelectrodes exhibited linearity for TNF-α in the 100 pg/mL-100 ng/mL range when measured in spiked serum, with limit of detection of 78 pg/mL. The sensor showed insignificant signal disturbance from serum proteins and other biologically important proteins. The developed platform was found to be fast and specific and can be applicable for testing and measuring various biologically important protein markers in real samples.

摘要

一种电化学酶联免疫吸附测定（ELISA）生物传感器平台，使用电化学制备的约 11nm 厚的羧酸功能化吡咯膜，用于未经稀释的血清中的生物分析物测量。使用 3-羧基-吡咯单体的羧基化聚吡咯（PPy-COOH）薄膜通过循环伏安法（CV）在梳状金电极微阵列（Au）上制备。然后，通过固定分析物特异性抗体，将制备的 Au/PPy-COOH 用于电化学 ELISA 平台的开发。选择肿瘤坏死因子-α（TNF-α）作为模型分析物，并在未经稀释的血清中进行检测。为了提高性能，研究了使用聚合碱性磷酸酶标签进行电化学 ELISA 的情况。在制造的每个步骤中，使用 CV、电化学阻抗谱和原子力显微镜对生物电极进行了表征。当在加标血清中测量时，该生物传感器在 100pg/mL-100ng/mL 范围内对 TNF-α表现出线性，检测限为 78pg/mL。该传感器显示出对血清蛋白和其他重要生物蛋白的信号干扰不显著。该开发的平台被发现快速且具有特异性，可适用于测试和测量实际样品中各种重要的生物蛋白标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/73d71f614235/sensors-20-02857-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/9cd9601e4a57/sensors-20-02857-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/2fe0d423d69d/sensors-20-02857-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/f53acaa35337/sensors-20-02857-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/c674c2586dcd/sensors-20-02857-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/5e36ca541b4a/sensors-20-02857-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/73d71f614235/sensors-20-02857-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/9cd9601e4a57/sensors-20-02857-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/2fe0d423d69d/sensors-20-02857-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/f53acaa35337/sensors-20-02857-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/c674c2586dcd/sensors-20-02857-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/5e36ca541b4a/sensors-20-02857-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c232/7287672/73d71f614235/sensors-20-02857-g006.jpg

相似文献

1
Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platform.基于聚吡咯的平台在未稀释血清中进行电化学 ELISA 蛋白质生物传感
Sensors (Basel). 2020 May 18;20(10):2857. doi: 10.3390/s20102857.
2
Electrochemical immunosensor for tumor necrosis factor-alpha detection in undiluted serum.用于检测未稀释血清中肿瘤坏死因子-α的电化学免疫传感器。
Methods. 2017 Mar 1;116:125-131. doi: 10.1016/j.ymeth.2016.12.001. Epub 2016 Dec 10.
3
Biocompatible epoxysilane substituted polymer-based nano biosensing platform for label-free detection of cancer biomarker SP17 in patient serum samples.基于生物相容的环氧硅烷取代聚合物的纳米生物传感平台，用于无标记检测患者血清样本中的癌症生物标志物 SP17。
Int J Biol Macromol. 2023 Jun 1;239:124325. doi: 10.1016/j.ijbiomac.2023.124325. Epub 2023 Apr 11.
4
High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film.基于壳聚糖-葡萄糖氧化酶固定化聚吡咯/ Nafion/功能化多壁碳纳米管生物纳米杂化膜的高性能葡萄糖生物传感器。
J Colloid Interface Sci. 2016 Nov 15;482:39-47. doi: 10.1016/j.jcis.2016.07.067. Epub 2016 Jul 27.
5
Reagentless impedimetric immunosensor for monitoring of methotrexate in human blood serum using multiwalled carbon nanotube@polypyrrole/polytyramine film electrode.无试剂电化学免疫传感器监测人血清中甲氨蝶呤使用多壁碳纳米管@聚吡咯/聚酪氨酸薄膜电极。
Talanta. 2024 Feb 1;268(Pt 1):125316. doi: 10.1016/j.talanta.2023.125316. Epub 2023 Oct 16.
6
Nanostructured sensor platform based on organic polymer conjugated to metallic nanoparticle for the impedimetric detection of SARS-CoV-2 at various stages of viral infection.基于有机聚合物与金属纳米粒子偶联的纳米结构传感器平台，用于在病毒感染的各个阶段进行 SARS-CoV-2 的阻抗检测。
J Pharm Biomed Anal. 2021 Nov 30;206:114392. doi: 10.1016/j.jpba.2021.114392. Epub 2021 Sep 24.
7
Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing.离子液体-聚吡咯-金复合材料作为增强的酶固定化平台用于过氧化氢传感。
Sensors (Basel). 2019 Feb 3;19(3):640. doi: 10.3390/s19030640.
8
Fabrication and characterisation of high performance polypyrrole modified microarray sensor for ascorbic acid determination.用于测定抗坏血酸的高性能聚吡咯修饰微阵列传感器的制作与特性研究。
Anal Chim Acta. 2013 Sep 2;793:11-8. doi: 10.1016/j.aca.2013.06.049. Epub 2013 Jul 6.
9
Flexible sensor based on conducting polymer and gold nanoparticles for electrochemical screening of HPV families in cervical specimens.基于导电聚合物和金纳米粒子的柔性传感器，用于电化学筛选宫颈标本中的 HPV 家族。
Talanta. 2021 May 1;226:122118. doi: 10.1016/j.talanta.2021.122118. Epub 2021 Jan 21.
10
Off surface matrix based on-chip electrochemical biosensor platform for protein biomarker detection in undiluted serum.基于离表面基质的片上电化学生物传感器平台，用于检测未稀释血清中的蛋白质生物标志物。
Biosens Bioelectron. 2017 Jun 15;92:542-548. doi: 10.1016/j.bios.2016.10.063. Epub 2016 Oct 27.

引用本文的文献

1
Anti-Fouling Strategies of Electrochemical Sensors for Tumor Markers.电化学传感器用于肿瘤标志物的抗污策略。
Sensors (Basel). 2023 May 30;23(11):5202. doi: 10.3390/s23115202.
2
Advanced wearable biosensors for the detection of body fluids and exhaled breath by graphene.基于石墨烯的用于检测体液和呼气的先进可穿戴生物传感器。
Mikrochim Acta. 2022 May 28;189(6):236. doi: 10.1007/s00604-022-05317-2.
3
Rapid Preparation of Fluorescent Carbon Dots from Pine Needles for Chemical Analysis.从松针中快速制备用于化学分析的荧光碳点

本文引用的文献

1
Cubic CeO implanted reduced graphene oxide-based highly sensitive biosensor for non-invasive oral cancer biomarker detection.用于无创口腔癌生物标志物检测的立方氧化铈植入还原氧化石墨烯基高灵敏度生物传感器。
J Mater Chem B. 2018 May 21;6(19):3000-3012. doi: 10.1039/c8tb00653a. Epub 2018 Apr 25.
2
Ultrasensitive Immunosensor Array for TNF-α Detection in Artificial Saliva using Polymer-Coated Magnetic Microparticles onto Screen-Printed Gold Electrode.基于聚合物涂层磁性微球和丝网印刷金电极的用于人工唾液中 TNF-α 检测的超灵敏免疫传感器阵列
Sensors (Basel). 2019 Feb 8;19(3):692. doi: 10.3390/s19030692.
3
Calorimetric sandwich-type immunosensor for quantification of TNF-α.
Nanomaterials (Basel). 2021 Dec 28;12(1):66. doi: 10.3390/nano12010066.
4
Non-Invasive Electrochemical Biosensors for TNF-α Cytokines Detection in Body Fluids.用于检测体液中肿瘤坏死因子-α细胞因子的无创电化学生物传感器
Front Bioeng Biotechnol. 2021 Sep 21;9:701045. doi: 10.3389/fbioe.2021.701045. eCollection 2021.
5
Electrochemical Biosensors for Cytokine Profiling: Recent Advancements and Possibilities in the Near Future.电化学生物传感器用于细胞因子分析：近期进展及未来展望。
Biosensors (Basel). 2021 Mar 23;11(3):94. doi: 10.3390/bios11030094.
用于定量检测 TNF-α 的量热三明治型免疫传感器。
Biosens Bioelectron. 2019 Feb 1;126:82-87. doi: 10.1016/j.bios.2018.10.028. Epub 2018 Oct 22.
4
Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection.电化学 ELISA 免疫分析用于癌症生物标志物检测的增强策略的最新进展。
Sensors (Basel). 2018 Jun 22;18(7):2010. doi: 10.3390/s18072010.
5
Electrochemical immunosensor for highly sensitive and quantitative detection of tumor necrosis factor-α in human serum.电化学免疫传感器用于人血清中肿瘤坏死因子-α的高灵敏度和定量检测。
Bioelectrochemistry. 2018 Aug;122:93-102. doi: 10.1016/j.bioelechem.2018.03.007. Epub 2018 Mar 20.
6
Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection.基于叉指电极的敏感和选择性 Affimer 功能化电容生物传感器，用于 Her4 蛋白肿瘤生物标志物检测。
Biosens Bioelectron. 2018 Jun 15;108:1-8. doi: 10.1016/j.bios.2018.02.041. Epub 2018 Feb 21.
7
Electrochemical immunosensors - A powerful tool for analytical applications.电化学免疫传感器 - 分析应用的有力工具。
Biosens Bioelectron. 2018 Apr 15;102:470-478. doi: 10.1016/j.bios.2017.11.029. Epub 2017 Nov 8.
8
A low-cost and miniaturized potentiostat for sensing of biomolecular species such as TNF-α by electrochemical impedance spectroscopy.一种低成本、微型化的电化学阻抗谱传感器，用于检测生物分子物种，如 TNF-α。
Biosens Bioelectron. 2018 Feb 15;100:533-540. doi: 10.1016/j.bios.2017.09.049. Epub 2017 Sep 28.
9
Recent advances in biosensor technology in assessment of early diabetes biomarkers.生物传感器技术在早期糖尿病生物标志物评估中的最新进展。
Biosens Bioelectron. 2018 Jan 15;99:122-135. doi: 10.1016/j.bios.2017.07.047. Epub 2017 Jul 19.
10
A highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNFα in human saliva and serum samples.基于 ITO 薄膜的高灵敏度免疫传感器，其表面覆盖有新型半导共轭聚合物，用于测定人唾液和血清样品中的 TNFα。
Biosens Bioelectron. 2017 Nov 15;97:169-176. doi: 10.1016/j.bios.2017.05.056. Epub 2017 Jun 2.