College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China; Guizhou Animal Husbandry and Veterinary Institute, Guiyang, Guizhou, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China; Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, China.
Poult Sci. 2023 Mar;102(3):102482. doi: 10.1016/j.psj.2023.102482. Epub 2023 Jan 6.
Sodium dehydroacetate (S-DHA) is used widely as a preservative in several products, including poultry feed. The anticoagulation effect of 200 mg/kg S-DHA in rats has been reported to accompany a reduction in hepatic expression of vitamin K epoxide reductase complex 1 (VKORC1). Poultry and mammals have different physiology and coagulation systems, and species differences in VKORC1 expression have been found. The effect of S-DHA on blood clotting of poultry has not been studies deeply. S-DHA was given to yellow-plumage broilers (YBs) as single and multiple administrations. Vitamin K3 (VK) was injected into YBs 2 wk after S-DHA administration. Then, the prothrombin time (PT), partial activated prothrombin time (APTT), plasma levels of vitamin K (VK), factor IX (FIX), and S-DHA, and hepatic expression of VKORC1 were obtained. Chicken hepatocellular carcinoma (LMH) cells were also exposed to S-DHA, and the cell activity, VK level, and FIX level were measured. S-DHA prolonged the PT or APTT significantly, decreased levels of VK and FIX in blood, and inhibited hepatic expression of VKORC1. The maximum changes were 1.15-fold in the PT, 1.42-fold in the APTT, 0.8-fold in the VK level, 0.7-fold in the FIX level, and 0.35-fold in VKORC1 expression compared with controls. The cell activity, VK level, FIX level, and VKORC1/VKORC1L1 expression of LMH cells were reduced significantly at S-DHA doses of 2.0 to 10.0 mM. Prolongation of the PT/APTT and lower levels of VK/FIX in YBs or the lower cell activity and VK/FIX levels in LMH cells induced by S-DHA therapy were resisted significantly by VK treatment. We demonstrated that S-DHA could induce a disorder in coagulation function in YBs or in LMH cells via reduction of VKORC1/VKORC1L1 expression, and that VK could resist this anticoagulation effect.
脱氢乙酸钠（S-DHA）被广泛用作几种产品（包括家禽饲料）中的防腐剂。据报道，200mg/kg S-DHA 可使大鼠的抗凝效果伴随肝维生素 K 环氧化物还原酶复合物 1（VKORC1）表达减少。家禽和哺乳动物具有不同的生理和凝血系统，并且已经发现 VKORC1 表达存在种间差异。S-DHA 对家禽凝血的影响尚未深入研究。S-DHA 被给予黄羽肉鸡（YBs）作为单次和多次给药。S-DHA 给药 2 周后，向 YBs 注射维生素 K3（VK）。然后，获得凝血酶原时间（PT）、部分活化凝血酶原时间（APTT）、血浆维生素 K（VK）、因子 IX（FIX）和 S-DHA 水平以及肝 VKORC1 表达。鸡肝癌细胞（LMH）也暴露于 S-DHA，并且测量细胞活性、VK 水平和 FIX 水平。S-DHA 显著延长 PT 或 APTT，降低血液中 VK 和 FIX 水平，并抑制肝 VKORC1 表达。与对照组相比，PT 最大变化为 1.15 倍，APTT 最大变化为 1.42 倍，VK 水平最大变化为 0.8 倍，FIX 水平最大变化为 0.7 倍，VKORC1 表达最大变化为 0.35 倍。当 S-DHA 剂量为 2.0 至 10.0mM 时，LMH 细胞的细胞活性、VK 水平、FIX 水平和 VKORC1/VKORC1L1 表达显著降低。S-DHA 治疗诱导的 YBs 中 PT/APTT 延长和 VK/FIX 水平降低或 LMH 细胞中细胞活性和 VK/FIX 水平降低，通过 VK 处理可显著抵抗。我们证明 S-DHA 可以通过降低 VKORC1/VKORC1L1 表达来诱导 YBs 或 LMH 细胞中的凝血功能障碍，并且 VK 可以抵抗这种抗凝作用。
