超高效液相色谱法同时快速分析加工动物产品中的化学防腐剂
Simultaneous and rapid analysis of chemical preservatives in processed animal products by ultra-performance liquid chromatography.
作者信息
Park Jwahaeng, Choi Sunju, Oh Donghwan, Mah Jae-Hyung
机构信息
1Ministry of Food and Drug Safety, Cheongju, Chungcheongbuk-do 28159 Republic of Korea.
2Department of Biotechnology, Korea University, Seoul, 02841 Republic of Korea.
出版信息
Food Sci Biotechnol. 2017 Dec 12;27(1):291-298. doi: 10.1007/s10068-017-0238-4. eCollection 2018 Feb.
An ultra-performance liquid chromatography-tunable ultraviolet method was optimized and validated for the simultaneous analysis of nine chemical preservatives in processed animal products. The limits of detection and quantification for the preservatives were within the ranges of 0.02-0.23 and 0.07-0.76 μg/mL, respectively. The relative standard deviations for intraday analyses of retention time and peak area were 0.00-0.23 and 0.03-2.93%, respectively, whereas, those for interday analyses were 0.67-2.30 and 2.12-5.37%, respectively. Of the nine preservatives spiked into six different animal products, dehydroacetic acid spiked into soft cheese exhibited the lowest recovery rate of 72.1 ± 0.36% at the lowest concentration (0.25 g/kg). Comparing data between UPLC and high-performance liquid chromatography with a 5% significance level, the t-statistic was 1.42. Moreover, sorbic acid was detected in 16 animal products (0.11-2.49 g/kg) when 278 products were analyzed for preservatives.
优化并验证了一种超高效液相色谱 - 可调紫外方法，用于同时分析加工动物产品中的九种化学防腐剂。这些防腐剂的检测限和定量限分别在0.02 - 0.23和0.07 - 0.76μg/mL范围内。保留时间和峰面积日内分析的相对标准偏差分别为0.00 - 0.23%和0.03 - 2.93%，而日间分析的相对标准偏差分别为0.67 - 2.30%和2.12 - 5.37%。在六种不同动物产品中添加的九种防腐剂中，添加到软奶酪中的脱氢乙酸在最低浓度（0.25 g/kg）时回收率最低，为72.1±0.36%。在5%的显著性水平下比较超高效液相色谱和高效液相色谱的数据，t统计量为1.42。此外，在分析278种产品中的防腐剂时，在16种动物产品中检测到山梨酸（0.11 - 2.49 g/kg）。
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