Laboratory of Nutritional Genomics, University of Campinas (UNICAMP), Limeira, São Paulo, Brazil; Nutrigenomics and Lipids Research Center (CELN), University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.
Nutrigenomics and Lipids Research Center (CELN), University of Campinas (UNICAMP), Limeira, São Paulo, Brazil; Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.
J Nutr Biochem. 2023 Apr;114:109270. doi: 10.1016/j.jnutbio.2023.109270. Epub 2023 Jan 25.
It is known that long-term high-fat diet (HF) feeding drastically affects the adipose tissue, contributing to metabolic disorders. Recently, short-term HF consumption was shown to affect different neuronal signaling pathways. Thus, we aimed to evaluate the inflammatory effects of a short-term HF and whether a diet containing omega-3 fatty acid fats from flaxseed oil (FS) has protective effects. Mice were divided into three groups for 3 d, according to their diets: Control group (CT), HF, or FS for 3 d. Lipid profiles were assessed through mass spectrometry and inflammatory markers by RT-qPCR and Western blotting. After short-term HF, mice increased food intake, body weight, adiposity, and fasting glucose. Increased mRNA content of Ccl2 and Tnf was demonstrated in the HF compared to CT in mesenteric adipose tissue. In the liver, TNFα protein was higher in the HF group than in CT, followed by a decreased polyunsaturated fatty acids tissue incorporation in HF. On the other hand, the consumption of FS reduced food intake and fasting glucose, as well as increased omega-3 fatty acid incorporation in MAT and the liver. However, short-term FS was insufficient to control the early inflammation triggered by HF in MAT and the liver. These data demonstrated that a 3-d HF diet is enough to damage glucose homeostasis and trigger inflammation. In contrast, short-term FS protects against increased food intake and fasting glucose but not inflammation in mice.
已知长期高脂肪饮食（HF）会严重影响脂肪组织，导致代谢紊乱。最近，短期 HF 消费被证明会影响不同的神经元信号通路。因此，我们旨在评估短期 HF 的炎症作用，以及是否含有亚麻籽油（FS）ω-3 脂肪酸的饮食具有保护作用。将小鼠分为三组，根据饮食分为 3 天：对照组（CT）、HF 或 FS 组 3 天。通过质谱法评估脂质谱，通过 RT-qPCR 和 Western blot 评估炎症标志物。在短期 HF 后，与 CT 相比，HF 组小鼠的食物摄入量、体重、肥胖和空腹血糖增加。与 CT 相比，HF 组肠系膜脂肪组织中 Ccl2 和 Tnf 的 mRNA 含量增加。在肝脏中，HF 组的 TNFα 蛋白高于 CT 组，随后 HF 组多不饱和脂肪酸组织的掺入减少。另一方面，FS 的消耗减少了食物摄入和空腹血糖，同时增加了 MAT 和肝脏中 ω-3 脂肪酸的掺入。然而，短期 FS 不足以控制 MAT 和肝脏中 HF 引发的早期炎症。这些数据表明，3 天 HF 饮食足以损害葡萄糖稳态并引发炎症。相比之下，短期 FS 可预防小鼠食物摄入增加和空腹血糖升高，但不能预防炎症。
