Lu Q, Zhang J N, Huo Y, Xia Q, Jiao J Y, Li M
Department of Otolaryngology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2022 Dec 7;57(12):1479-1483. doi: 10.3760/cma.j.cn115330-20220322-00125.
The susceptibility of tinnitus rats with low estrogen level induced by sodium salicylate and the changes of tumor necrosis factor α (TNF-α) in serum were observed to investigate the relationship between tinnitus occurrence and estrogen level. Forty-two healthy female Wistar rats were randomly divided into control group(=6), normal group(=6), sham operation group(=6) and ovariectomized group(=24). Control group was intraperitoneally injected with normal saline 200 mg/kg for 14 consecutive days. Normal group, sham operation group and ovariectomized group were intraperitoneally injected with sodium salicylate 200 mg/kg for 14 consecutive days. Before and after sodium salicylate induction, the tinnitus behavior of rats in each group was detected by prepulse inhibition (PPI) and gap pre-pulse inhibition of the acoustic startle (GPIAS) test. Before and after sodium salicylate induction, blood samples were collected from eyeballs of rats in each group, and serum levels of estradiol and TNF-α were detected by ELISA. SPSS 25.0 software was used to analyze the data. (1) Following 14 days of sodium salicylate intervention, there was no significant difference in PPI inhibition rate between groups or within groups(all >0.05). (2)There was no significant difference in the inhibition rate of GPIAS in the four groups before sodium salicylate injection(=0.217, >0.05). With sodium salicylate injected for 14 days, the inhibition rate of GPIAS in ovariectomized group (30.88%±15.40%) was significantly lower than that in the other three groups (44.11%±21.06%, 38.27%±10.92%, 51.59%±11.34%), and the difference was statistically significant(=3.533, <0.05). The inhibition rate of GPIAS in ovariectomized group with sodium salicylate injected for 14 days was significantly lower than that before injection, and the difference was statistically significant(=2.977, <0.05).There was no significant difference in GPIAS inhibition rate between the other three groups before and after sodium salicylate injection(>0.05). (3)The level of TNF-α in ovariectomized rats was significantly higher than that in the other three groups, the difference was statistically significant(all <0.05). With sodium salicylate injection for 14 days, TNF-α level in the ovariectomized group increased more significantly than that in the other three groups, the difference was statistically significant(=8.045, <0.05). TNF-α levels increased following salicylate injection in normal group, sham operation group and ovariectomized group, and the differences were statistically significant( value was -4.843, -4.932 and -5.965 respectively, each <0.05). There was no significant difference in TNF-α levels before and after normal saline injection in control group(all >0.05). Low estrogen levels increase susceptibility to sodium salicylate-induced tinnitus. Decreased estrogen levels may increase susceptibility to tinnitus through the increased expression of pro-inflammatory factor TNF-α.
观察水杨酸钠诱导的低雌激素水平耳鸣大鼠的易感性及血清肿瘤坏死因子α（TNF-α）的变化，以探讨耳鸣发生与雌激素水平的关系。将42只健康雌性Wistar大鼠随机分为对照组（=6）、正常组（=6）、假手术组（=6）和去卵巢组（=24）。对照组连续14天腹腔注射生理盐水200mg/kg。正常组、假手术组和去卵巢组连续14天腹腔注射水杨酸钠200mg/kg。在水杨酸钠诱导前后，通过预脉冲抑制（PPI）和声惊吓间隙预脉冲抑制（GPIAS）试验检测各组大鼠的耳鸣行为。在水杨酸钠诱导前后，从各组大鼠眼球采集血样，采用ELISA法检测血清雌二醇和TNF-α水平。使用SPSS 25.0软件进行数据分析。（1）水杨酸钠干预14天后，组间及组内PPI抑制率差异均无统计学意义（均>0.05）。（2）注射水杨酸钠前，四组GPIAS抑制率差异无统计学意义（=0.217，>0.05）。注射水杨酸钠14天后，去卵巢组GPIAS抑制率（30.88%±15.40%）显著低于其他三组（44.11%±21.06%、38.27%±10.92%、51.59%±11.34%），差异有统计学意义（=3.533，<0.05）。注射水杨酸钠14天的去卵巢组GPIAS抑制率显著低于注射前，差异有统计学意义（=2.977，<0.05）。其他三组注射水杨酸钠前后GPIAS抑制率差异无统计学意义（>0.05）。（3）去卵巢大鼠TNF-α水平显著高于其他三组，差异有统计学意义（均<0.05）。注射水杨酸钠14天，去卵巢组TNF-α水平升高幅度显著大于其他三组，差异有统计学意义（=8.045，<0.05）。正常组、假手术组和去卵巢组注射水杨酸钠后TNF-α水平均升高，差异有统计学意义（值分别为-4.843、-4.932和-5.965，均<0.05）。对照组注射生理盐水前后TNF-α水平差异无统计学意义（均>0.05）。低雌激素水平增加了对水杨酸钠诱导耳鸣的易感性。雌激素水平降低可能通过促炎因子TNF-α表达增加而增加耳鸣易感性。
