CPHEN-15：通过流式细胞术对人外周血辅助性 ILC 进行全面表型分析。
CPHEN-15: Comprehensive phenotyping of human peripheral blood helper-ILCs by flow cytometry.
机构信息
Istituto Giannina Gaslini, IRCCS, Genoa, Italy.
Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
出版信息
Cytometry A. 2023 May;103(5):378-382. doi: 10.1002/cyto.a.24717. Epub 2023 Jan 28.
Innate lymphoid cells (ILCs) comprise cytotoxic NK cells and helper-ILCs, which are further divided in ILC1, ILC2, and ILC3. Helper-ILCs mirror the effector functions of helper T-cell subsets and contribute to host immune defense, tissue homeostasis and repair through cytokine secretion. Although they are mainly tissue-resident, helper ILCs are also found in the peripheral blood (PB). In the human setting, it may be needed to analyze circulating helper ILCs to compare pathological to physiological conditions. In this review, we provide simple guidelines and a list of markers useful to study human PB helper ILCs phenotype and function by flow cytometry.
先天淋巴细胞 (ILC) 包括细胞毒性 NK 细胞和辅助性 ILC，后者又进一步分为 ILC1、ILC2 和 ILC3。辅助性 ILC 通过细胞因子分泌反映辅助性 T 细胞亚群的效应功能，并有助于宿主免疫防御、组织稳态和修复。尽管它们主要存在于组织中，但辅助性 ILC 也存在于外周血 (PB) 中。在人类环境中，可能需要分析循环辅助性 ILC 以比较病理与生理条件。在这篇综述中，我们提供了简单的指南和标记物列表，用于通过流式细胞术研究人类 PB 辅助性 ILC 的表型和功能。
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