Maqsood Maha, Sharif Saima, Naz Shagufta, Farasat Tasnim, Manzoor Farkhanda, Cheema Maqsood, Saqib Muhammad
Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
DHQ Teaching Hospital Gujranwala, Civil Lines, Gujranwala, Pakistan.
Mol Biol Rep. 2023 Apr;50(4):3249-3257. doi: 10.1007/s11033-023-08249-z. Epub 2023 Jan 28.
Diabetic nephropathy (DN) is a micro-chronic diabetic consequence induced by metabolic and hemodynamic abnormalities. Free radicals react with other critical cellular components, causing progression of aberrant renal function.
This case control study was aimed to determine the role of IL-6 and IL-18 in diabetic nephropathy in Pakistani population.
The study's subjects (n = 180 from Lahore, Gujranwala, and Karachi) were divided into control, diabetes mellitus (DM) and diabetic nephropathy (DN) groups. The serum concentration of IL-6 & IL-18 were determined by enzyme-linked immunosorbent assay (ELISA). The expression analysis of IL-6 & IL-18 were performed by Real Time PCR.
The significant increase in serum levels of IL-6 were observed among the control, DM and DN groups (15.3 ± 24.1 pg/ml, 34.7 ± 24.0 pg/ml, 52.6 ± 33.2 pg/ml) whereas no significant difference was observed in serum levels of IL-18. The expression analysis of IL-6 was increased by more than forty three fold in DN group (n-fold = ~43.6) as compared to DM & control whereas the expression profile of IL-18 decreased in DN group (n-fold = ~0.89). In DN group the correlation analysis revealed direct association of GFR with serum IL-6 (r = 0.1114) & inverse relationship with serum IL-18 (r = - 0.097). In multiple regression analysis using GFR as the dependent variable, BMI and expression of IL-18 were determinants in DM subjects, but only uric acid in DN subjects.
The present study implicates that increased expression of IL-6 and decreased of IL-18 was associated with development of DN in Pakistani population.
糖尿病肾病（DN）是一种由代谢和血流动力学异常引起的慢性糖尿病微血管并发症。自由基与其他关键细胞成分发生反应，导致肾功能异常进展。
本病例对照研究旨在确定白细胞介素-6（IL-6）和白细胞介素-18（IL-18）在巴基斯坦人群糖尿病肾病中的作用。
研究对象（来自拉合尔、古杰兰瓦拉和卡拉奇的180例）分为对照组、糖尿病（DM）组和糖尿病肾病（DN）组。采用酶联免疫吸附测定（ELISA）法测定血清IL-6和IL-18浓度。通过实时荧光定量聚合酶链反应（Real Time PCR）进行IL-6和IL-18的表达分析。
对照组、DM组和DN组血清IL-6水平显著升高（分别为15.3±24.1 pg/ml、34.7±24.0 pg/ml、52.6±33.2 pg/ml），而血清IL-18水平无显著差异。与DM组和对照组相比，DN组IL-6的表达分析增加了43倍以上（n倍=43.6），而DN组IL-18的表达谱降低（n倍=0.89）。在DN组中，相关性分析显示肾小球滤过率（GFR）与血清IL-6呈正相关（r=0.1114），与血清IL-18呈负相关（r=-0.097）。在以GFR为因变量的多元回归分析中，BMI和IL-18的表达是DM患者的决定因素，而在DN患者中只有尿酸是决定因素。
本研究表明，IL-6表达增加和IL-18表达降低与巴基斯坦人群DN的发生有关。
