Faculty of Pharmacy, Damghan Branch, Islamic Azad University, Damghan, Iran.
Research Institute for Persian and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2023 Jan 1;24(1):275-281. doi: 10.31557/APJCP.2023.24.1.275.
Melanoma is a malignant tumor that originates from the skin's melanocytes and has the highest death rate from skin cancer. Developing more efficacious anticancer medications with fewer adverse effects is the key to effective cancer management. Natural products are considered relevant and cost-effective sources of treatment. The plant (Polypodium vulgare) is a small and evergreen fern. One of the most important chemical compounds in the extract of this herb is flavonoids, which are thought to have beneficial effects in the treatment of melanoma through antioxidant properties.
Due to the limitations of current cancer management and cytotoxic drugs available in the country, the need to study drugs of natural origin has become more prominent. In this regard, the present study aims to investigate the cytotoxic effects of the ethanolic extract of Polypodium vulgare on A375 melanoma cells.
Polypodium vulgare was extracted in 80% ethanol by the maceration. Then, its effects on the cell death of the melanoma cell line A375 compared to the AGO-1522 cell line as control were measured using the MTT-assay technique. The amount of cellular lipid peroxidation was estimated by TBARS assay. The amount of cellular ROS was calculated by fluorescent reagent 2,7-dichlorofluorescein diacetate. Cytochrome c concentration was measured by a cytochrome c immunoassay kit.
In this experiment, the anticancer effects of Polypodium vulgare ethanolic extract on human melanoma cell lines were investigated for the first time. Herb extract with a concentration of 0.123 mg/ml significantly increased the death of A375 melanoma cells (p < 0.001), lipid peroxidation (p < 0.01), and reactive oxygen species (ROS) (p < 0.01) and cytochrome c concentration (p < 0.001). Meanwhile, the same amount was ineffective and safe on AGO-1522 normal fibroblast cells.
A 0.123 mg/ml concentration of Polypodium vulgare increases apoptosis in melanoma cells. Meanwhile, the same amount was safe on healthy cells. So, it could be considered an effective treatment without side effects in human melanoma.
黑色素瘤是一种起源于皮肤黑素细胞的恶性肿瘤，是皮肤癌中死亡率最高的肿瘤。开发更有效且副作用更少的抗癌药物是有效癌症管理的关键。天然产物被认为是相关且具有成本效益的治疗来源。该植物（Polypodium vulgare）是一种小型的常绿蕨类植物。这种草药提取物中最重要的化合物之一是类黄酮，它被认为具有抗氧化特性，可通过抗氧化特性对治疗黑色素瘤有益。
由于目前癌症管理的局限性以及国内可用的细胞毒性药物，对天然来源药物的研究变得更加突出。在这方面，本研究旨在研究 Polypodium vulgare 的乙醇提取物对 A375 黑色素瘤细胞的细胞毒性作用。
用浸渍法用 80%乙醇从 Polypodium vulgare 中提取，然后使用 MTT 测定法测量其对黑色素瘤细胞系 A375 的细胞死亡与对照 AGO-1522 细胞系的影响。通过 TBARS 测定法估计细胞内脂质过氧化的量。通过荧光试剂 2,7-二氯荧光素二乙酸酯计算细胞内 ROS 的量。通过细胞色素 c 免疫测定试剂盒测量细胞色素 c 浓度。
在这项实验中，首次研究了 Polypodium vulgare 乙醇提取物对人黑色素瘤细胞系的抗癌作用。浓度为 0.123mg/ml 的草药提取物可显著增加 A375 黑色素瘤细胞的死亡（p <0.001），脂质过氧化（p <0.01），活性氧（ROS）（p <0.01）和细胞色素 c 浓度（p <0.001）。同时，相同剂量对 AGO-1522 正常成纤维细胞无效且安全。
浓度为 0.123mg/ml 的 Polypodium vulgare 可增加黑色素瘤细胞的凋亡。同时，相同剂量对健康细胞是安全的。因此，它可以被认为是一种有效的治疗方法，没有副作用，对人类黑色素瘤有效。
