Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh; School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Biomater Adv. 2023 Mar;146:213291. doi: 10.1016/j.bioadv.2023.213291. Epub 2023 Jan 18.
The phytochemicals of Tamarindus indica seed hydroalcoholic extract were exploited as a biocatalyst for the sustainable synthesis of magnesium oxide nanoparticles (MgO-NPs). This research investigated the cardioprotective effects of biosynthesized magnesium oxide nanoparticle (MgO-NPs). The biosynthesized seed MgO-NPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy-dispersive X-ray diffraction (EDX), and Fourier-transform infrared spectroscopy (FT-IR). These methodological approaches demonstrated their capacity to synthesize crystalline and aggregated MgO-NPs with a size average of 13.38 ± 0.16 nm. The biogenic MgO-NPs were found to have a significant quantity of total phenolic contents (TPC) and total flavonoid contents (TFC), indicating the existence of phenol and flavonoid-like components. The biogenic MgO-NPs demonstrated a significant free radical scavenging effects compared to different standards as measured by the inhibition of free radicals produced in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and Nitric oxide (NO) scavenging methods; they also exhibited higher ferric ion reducing capacity in FRAP assay. Moreover, they were found to be non-toxic in cytotoxic assessment. Pretreatment of Wistar Albino rats with seed MgO-NPs resulted in a significant reduction of cardiac biomarkers, i.e., cardiac Troponin-I (cTnI), creatine kinase (CK-MB), and aspartate aminotransferase (AST). The seed MgO-NPs were more successful in reducing lipid levels. The results of the mRNA expression analysis showed that seed MgO-NPs efficiently reduced the expression of the apoptotic genes p53 and Caspase-3 while restoring the expected levels of SOD gene expression. The histopathological observations were primarily focused on the disruption of cardiac fibers and myofibrillar disintegration, which are consistent with the biochemical findings. Therefore, our research suggests that MgO-NPs derived from the seeds of Tamarindus indica as a powerful antioxidant; the administration may be effective in protecting the heart from DOX-induced cardiotoxicity.
罗望子种子水醇提取物的植物化学成分被用作生物催化剂，用于可持续合成氧化镁纳米粒子（MgO-NPs）。本研究调查了生物合成氧化镁纳米粒子（MgO-NPs）的心脏保护作用。生物合成的种子 MgO-NPs 通过紫外-可见光谱（UV-Vis）、X 射线衍射（XRD）、扫描电子显微镜（SEM）与能量色散 X 射线衍射（EDX）和傅里叶变换红外光谱（FT-IR）进行了表征。这些方法学方法表明，它们能够合成结晶和聚集的 MgO-NPs，平均粒径为 13.38±0.16nm。生物合成的 MgO-NPs 被发现具有大量的总酚含量（TPC）和总类黄酮含量（TFC），表明存在酚类和类黄酮类成分。与不同标准相比，生物合成的 MgO-NPs 表现出显著的自由基清除效果，这可以通过抑制 2,2-二苯基-1-苦基肼（DPPH）、2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和一氧化氮（NO）产生的自由基来测量; 它们在 FRAP 测定中也显示出更高的铁离子还原能力。此外，在细胞毒性评估中发现它们没有毒性。用种子 MgO-NPs 预处理 Wistar 白化大鼠可显著降低心脏标志物，即心肌肌钙蛋白 I（cTnI）、肌酸激酶（CK-MB）和天门冬氨酸氨基转移酶（AST）。种子 MgO-NPs 在降低脂质水平方面更成功。mRNA 表达分析的结果表明，种子 MgO-NPs 有效地降低了凋亡基因 p53 和 Caspase-3 的表达，同时恢复了 SOD 基因表达的预期水平。组织病理学观察主要集中在心肌纤维的破坏和肌原纤维的解体上，这与生化发现一致。因此，我们的研究表明，罗望子种子衍生的 MgO-NPs 作为一种强大的抗氧化剂; 这种给药方法可能对保护心脏免受 DOX 诱导的心脏毒性有效。
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