Fleming Hannah, Chamberlain Zachary, Zager Jordan J, Lange B Markus
Dewey Scientific LLC, Pullman, WA, United States.
Dewey Scientific LLC, Pullman, WA, United States; Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA, United States.
Methods Enzymol. 2023;680:353-380. doi: 10.1016/bs.mie.2022.07.028. Epub 2022 Sep 14.
The cannabis (Cannabis sativa L.) genome is highly heterozygous and, to retain genetic identity, clonal propagation of cultivars is very common. Establishing controlled environments, often involving multiple locations throughout a single grow, is critical for reliably generating materials to be used in research and production. In this article, we break down different periods of the grow cycle, such as cloning, hardening (optional), vegetative growth, flowering growth, and harvest, into individual steps. We are including images and videos for an in-depth coverage of methodological details. We are providing a list of equipment, supplies, reagents, and other resources to help with planning a grow experiment. Finally, we are discussing considerations for different aspects of controlled environments, including lighting, fertilizer regimes, and integrated pest management. With this article, it is our goal to empower researchers to reliably generate disease-free cannabis material suitable for genetic and biochemical studies that require full control of environmental factors.
大麻（Cannabis sativa L.）基因组高度杂合，为保持遗传特性，品种的克隆繁殖非常普遍。建立可控环境，通常在单一种植过程中涉及多个地点，对于可靠地生产用于研究和生产的材料至关重要。在本文中，我们将种植周期的不同阶段，如克隆、硬化（可选）、营养生长、花期生长和收获，分解为各个步骤。我们还提供了图像和视频，以深入涵盖方法细节。我们列出了设备、用品、试剂和其他资源清单，以帮助规划种植实验。最后，我们讨论了可控环境不同方面的注意事项，包括光照、施肥方案和综合虫害管理。通过本文，我们的目标是使研究人员能够可靠地生产出无病的大麻材料，适用于需要完全控制环境因素的遗传和生化研究。
