Chen Dawei, Liang Yating, Liang Jiaojiao, Shen Feifei, Cheng Yue, Qu Hengxian, Wa Yunchao, Guo Congcong, Gu Ruixia, Qian Jianya, Chen Xia, Zhang Chenchen, Guan Chengran
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou 225127, China; Jiangsu Yuhang Food Technology Co., Ltd., Yancheng 224200, China.
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou 225127, China.
J Dairy Sci. 2023 Mar;106(3):1533-1548. doi: 10.3168/jds.2022-22383. Epub 2023 Jan 27.
A growing stream of research suggests that probiotic fermented milk has a good effect on nonalcoholic fatty liver disease. This work aimed to study the beneficial effects of Lactobacillus rhamnosus hsryfm 1301 fermented milk (fermented milk) on rats with nonalcoholic fatty liver disease induced by a high-fat diet. The results showed that the body weight and the serum levels of total cholesterol, total glyceride, low-density lipoprotein, alanine transaminase, aspartate aminotransferase, free fatty acid, and reactive oxygen species were significantly increased in rats fed a high-fat diet (M) for 8 wk, whereas high-density lipoprotein cholesterol and superoxide dismutase were significantly decreased. However, the body weight and the serum levels of total cholesterol, total glyceride, alanine transaminase, aspartate aminotransferase, free fatty acid, reactive oxygen species, interleukin-8, tumor necrosis factor-α, and interleukin-6 were significantly decreased with fermented milk (T) for 8 wk, and the number of fat vacuoles in hepatocytes was lower than that in the M group. There were significant differences in 19 metabolites in serum between the M group and the C group (administration of nonfermented milk) and in 17 metabolites between the T group and the M group. The contents of 7 different metabolites, glycine, glycerophosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphocholine, thioetheramide-PC, d-aspartic acid, oleic acid, and l-glutamate, were significantly increased in the M group rat serum, and l-palmitoyl carnitine, N6-methyl-l-lysine, thymine, and 2-oxadipic acid were significantly decreased. In the T group rat serum, the contents of 8 different metabolites-1-O-(cis-9-octadecenyl)-2-O-acetyl-sn-glycero-3-phosphocholine, acetylcarnitine, glycine, glycerophosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphocholine, d-aspartic acid, oleic acid, and l-glutamate were significantly decreased, whereas creatinine and thymine were significantly increased. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 50 metabolic pathways were enriched in the M/C group and T/M group rat serum, of which 12 metabolic pathways were significantly different, mainly distributed in lipid metabolism, amino acid, and endocrine system metabolic pathways. Fermented milk ameliorated inflammation, oxygenation, and hepatocyte injury by regulating lipid metabolism, amino acid metabolic pathways, and related metabolites in the serum of rats with nonalcoholic fatty liver disease.
越来越多的研究表明，益生菌发酵乳对非酒精性脂肪性肝病有良好的效果。这项工作旨在研究鼠李糖乳杆菌hsryfm 1301发酵乳（发酵乳）对高脂饮食诱导的非酒精性脂肪性肝病大鼠的有益作用。结果表明，高脂饮食喂养8周的大鼠（M组）体重以及血清总胆固醇、总甘油三酯、低密度脂蛋白、丙氨酸转氨酶、天冬氨酸转氨酶、游离脂肪酸和活性氧水平显著升高，而高密度脂蛋白胆固醇和超氧化物歧化酶显著降低。然而，发酵乳喂养8周的大鼠（T组）体重以及血清总胆固醇、总甘油三酯、丙氨酸转氨酶、天冬氨酸转氨酶、游离脂肪酸、活性氧、白细胞介素-8、肿瘤坏死因子-α和白细胞介素-6水平显著降低，肝细胞内脂肪空泡数量低于M组。M组和给予未发酵乳的C组大鼠血清中有19种代谢物存在显著差异，T组和M组之间有17种代谢物存在显著差异。M组大鼠血清中7种不同代谢物，即甘氨酸、甘油磷酸胆碱、1,2-二油酰基-sn-甘油-3-磷酸胆碱、硫醚酰胺-PC、d-天冬氨酸、油酸和l-谷氨酸的含量显著增加，而l-棕榈酰肉碱、N6-甲基-l-赖氨酸、胸腺嘧啶和2-氧己二酸显著降低。在T组大鼠血清中，8种不同代谢物，即1-O-（顺式-9-十八碳烯基）-2-O-乙酰基-sn-甘油-3-磷酸胆碱、乙酰肉碱、甘氨酸、甘油磷酸胆碱、1,2-二油酰基-sn-甘油-3-磷酸胆碱、d-天冬氨酸、油酸和l-谷氨酸的含量显著降低，而肌酐和胸腺嘧啶显著增加。京都基因与基因组百科全书通路分析表明，M/C组和T/M组大鼠血清中有50条代谢通路富集，其中12条代谢通路存在显著差异，主要分布在脂质代谢、氨基酸和内分泌系统代谢通路中。发酵乳通过调节非酒精性脂肪性肝病大鼠血清中的脂质代谢、氨基酸代谢通路及相关代谢物，改善炎症、氧化应激和肝细胞损伤。
