Tümer Kenan Çağrı, Kızıl Meltem
Department of Internal Medicine, Kastamonu University, Faculty of Veterinary Medicine, Kastamonu, Türkiye.
Department of Physiology, Fırat University, Faculty of Veterinary Medicine, Elazığ, Türkiye.
Parasite Immunol. 2023 May;45(5):e12973. doi: 10.1111/pim.12973. Epub 2023 Feb 8.
The objective of the present study was to investigate the inflammatory and anti-inflammatory cytokine response from a broad perspective in cattle with natural Theileria annulata infection. Ten cattle naturally infected with T. annulata and eight healthy cattle were included in this study. A total of 11 cytokines, including tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, and IL-17 were evaluated in serum samples using commercially available enzyme-linked immunosorbent assay (ELISA) kits. There was no statistical significance for serum TNF-α, IL-1β, IL-5, IL-6, and IL-17 levels between the T. annulata infected and healthy cattle. In contrast, the median serum levels of IFN-γ (p = .023), IL-2 (p = .066), IL4 (p = .0016), IL-10 (p = .00087), IL-12 (p = .00018), and IL-13 (p = .023) were significantly higher in T. annulata-infected cattle than in healthy cattle. The results of the present study revealed that in the intraerythrocytic stage of tropical theileriosis, a very pronounced anti-inflammatory response occurs as well as an ongoing inflammatory process.
本研究的目的是从广泛的角度调查自然感染环形泰勒虫的牛体内的炎症和抗炎细胞因子反应。本研究纳入了10头自然感染环形泰勒虫的牛和8头健康牛。使用市售酶联免疫吸附测定（ELISA）试剂盒对血清样本中的11种细胞因子进行了评估，包括肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白细胞介素（IL）-1β、IL-2、IL-4、IL-5、IL-6、IL-10、IL-12、IL-13和IL-17。环形泰勒虫感染牛和健康牛之间的血清TNF-α、IL-1β、IL-5、IL-6和IL-17水平无统计学意义。相比之下，环形泰勒虫感染牛的血清IFN-γ（p = 0.023）、IL-2（p = 0.066）、IL4（p = 0.0016）、IL-10（p = 0.00087）、IL-12（p = 0.00018）和IL-13（p = 0.023）的中位数水平显著高于健康牛。本研究结果表明在热带泰勒虫病的红细胞内阶段，除了持续的炎症过程外，还会发生非常明显的抗炎反应。
