Preston P M, Brown C G, Entrican G, Richardson W, Boid R
Division of Biological Sciences, Ashworth Laboratories, University of Edinburgh, Scotland, UK.
Parasite Immunol. 1993 Sep;15(9):525-34. doi: 10.1111/j.1365-3024.1993.tb00640.x.
Bovine macrophage-derived tumour necrosis factor-alpha/cachectin (TNF-alpha) was synthesized when peripheral blood mononuclear cells (PBMC) and purified adherent PBMC from naive and Theileria annulata-infected cattle were incubated in vitro with concanavalin A (Con-A) or bovine recombinant interferon gamma (Bo rIFN-gamma). TNF-alpha production was also induced when adherent PBMC were cultured with T. annulata macroschizont-infected cells. In contrast, non-adherent PBMC from sublethally infected cattle produced interferon (IFN) when incubated with Hu rIL-2, Con-A, phytohaemagglutinin (PHA) or T. annulata macroschizont-infected cells growing as cell lines in vitro. Whilst PBMC from lethally infected cattle spontaneously produced IFN-gamma during advanced stages of infection, the sera of such animals contained type 1 IFN (alpha/beta). IFN was also produced by T. annulata macroschizont-infected cell lines maintained in vitro. This work suggests that cytokines serve as crucial links between proliferating Theileira-infected cells and the characteristic clinical symptoms of tropical theileriosis.
当用伴刀豆球蛋白A（Con-A）或牛重组干扰素γ（Bo rIFN-γ）在体外孵育来自未感染和环形泰勒虫感染牛的外周血单个核细胞（PBMC）以及纯化的贴壁PBMC时，可合成牛巨噬细胞衍生的肿瘤坏死因子-α/恶病质素（TNF-α）。当贴壁PBMC与环形泰勒虫大裂殖体感染的细胞一起培养时，也会诱导TNF-α的产生。相反，来自亚致死感染牛的非贴壁PBMC在与人类重组白细胞介素-2（Hu rIL-2）、Con-A、植物血凝素（PHA）或在体外作为细胞系生长的环形泰勒虫大裂殖体感染的细胞一起孵育时会产生干扰素（IFN）。虽然来自致死感染牛的PBMC在感染后期会自发产生IFN-γ，但这些动物的血清中含有1型干扰素（α/β）。体外培养的环形泰勒虫大裂殖体感染的细胞系也会产生IFN。这项工作表明，细胞因子是增殖的泰勒虫感染细胞与热带泰勒虫病特征性临床症状之间的关键联系。
