Ouyang Ming, Wang Guo-Hua
Department of Stomatology, Putuo Affiliated Hospital of Shanghai University of Traditional Chinese Medicine. Shanghai 200062, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Aug;31(4):379-383.
To explore the feasibility of adhesive thiomer calcium alginate composite hydrogel (ZIF-8@CHA-SH) containing metal-organic framework for the treatment of periodontitis in rats.
Preparation and characterization of ZIF-8@CHA-SH were performed, and the morphology and chemical composition were observed. The antibacterial properties were detected by plate coating method and scanning electron microscope. CCK-8 and live/dead cell fluorescence staining were used to evaluate the toxicity of ZIF-8@CHA-SH on gingival fibroblasts. The anti-inflammatory and tissue repair promoting effects of ZIF-8@CHA-SH were verified by animal experiments. GraphPad Prism 7.0 software was used for statistical analysis.
The successful synthesis of ZIF-8@CHA-SH was proved by scanning electron microscopy, energy spectrum analysis, Fourier transform infrared spectroscopy and X-ray diffraction. ZIF-8@CHA-SH had excellent antibacterial ability, and the antibacterial rates against Escherichia coli, Staphylococcus aureus and Streptococcus mutans were (99.88±0.12)%, (99.81±0.32)% and (95.53±3.08)%, respectively（P<0.001）. The cell viability rate of ZIF-8@CHA-SH was (91.64±3.66)% after 5 days of co-culture with human gingival fibroblasts (P=0.6). In vivo experiments showed that ZIF-8@CHA-SH could reduce the infiltration of inflammatory cells and the expression of pro-inflammatory factor IL-6 in rats with periodontitis.
ZIF-8@CHA-SH has excellent biological performance, which can effectively treat periodontitis caused by bacteria, and provids a new strategy for the treatment of periodontal diseases in the future.
探讨含金属有机框架的粘性硫醇海藻酸钙复合水凝胶（ZIF-8@CHA-SH）治疗大鼠牙周炎的可行性。
进行ZIF-8@CHA-SH的制备与表征，观察其形态和化学成分。采用平板涂布法和扫描电子显微镜检测抗菌性能。使用CCK-8和活/死细胞荧光染色评估ZIF-8@CHA-SH对牙龈成纤维细胞的毒性。通过动物实验验证ZIF-8@CHA-SH的抗炎和促进组织修复作用。使用GraphPad Prism 7.0软件进行统计分析。
通过扫描电子显微镜、能谱分析、傅里叶变换红外光谱和X射线衍射证明ZIF-8@CHA-SH成功合成。ZIF-8@CHA-SH具有优异的抗菌能力，对大肠杆菌、金黄色葡萄球菌和变形链球菌的抗菌率分别为(99.88±0.12)%、(99.81±0.32)%和(95.53±3.08)%（P<0.001）。与人牙龈成纤维细胞共培养5天后，ZIF-8@CHA-SH的细胞活力率为(91.64±3.66)%（P=0.6）。体内实验表明，ZIF-8@CHA-SH可减少牙周炎大鼠炎症细胞浸润和促炎因子IL-6的表达。
ZIF-8@CHA-SH具有优异的生物学性能，可有效治疗细菌引起的牙周炎，为未来牙周疾病的治疗提供了新策略。
