College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2024 Oct 1;42(5):593-608. doi: 10.7518/hxkq.2024.2024037.
This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis.
A thermosensitive hydrogel supported by carvacrol was prepared using poloxamer and hydroxypropyl methyl cellulose as matrix. SD rats were randomly divided into blank group, periodontitis group, blank hydrogel group, and low-, medium-, and high-dose hydrogel groups. The periodontitis symptoms and the CT structure of the alveolar bone were observed. The changes in liver, spleen, kidney, and periodontal tissues were observed. The related indexes of bone metabolism in serum were detected. The expression of osteoprotegerin (OPG) and nuclear transcription factor-κB (NF-κB) pathway proteins was determined by Western blot. The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
Carvacrol hydrogel had good slow release, biocompatibility, and cell adhesion. The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated, the expression of OPG protein in gingival tissue was significantly increased (<0.01), and the levels of receptor activator of NF-κB ligand (RANKL), receptor activator of NF-κB (RANK), NF-κB protein, and inflammatory factors were significantly decreased (<0.01).
Carvacrol hydrogel can regulate the OPG and NF-κB pathways, reduce alveolar bone absorption, and improve periodontal inflammation.
本研究旨在探讨香芹酚水凝胶对牙周炎大鼠牙槽骨的保护作用及其机制。
以泊洛沙姆和羟丙甲纤维素为基质，制备香芹酚水凝胶。SD 大鼠随机分为空白组、牙周炎组、空白水凝胶组、低、中、高剂量水凝胶组。观察牙周炎症状和牙槽骨 CT 结构，观察肝、脾、肾和牙周组织的变化，检测血清中骨代谢相关指标，采用 Western blot 法检测护骨素（OPG）和核转录因子-κB（NF-κB）通路蛋白的表达，采用定量逆转录聚合酶链反应（qRT-PCR）检测炎症因子水平。
香芹酚水凝胶具有良好的缓慢释放、生物相容性和细胞黏附性。香芹酚水凝胶组大鼠牙周炎明显缓解，牙龈组织中 OPG 蛋白表达明显增加（<0.01），核因子-κB 配体（RANKL）受体激活剂、核因子-κB（RANK）受体激活剂、NF-κB 蛋白和炎症因子水平明显降低（<0.01）。
香芹酚水凝胶可调节 OPG 和 NF-κB 通路，减少牙槽骨吸收，改善牙周炎炎症。
