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氨甲基三甲基补骨脂素与鸡输卵管mRNA光反应对卵清蛋白mRNA影响的表征。

Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.

作者信息

Liarakos C D, Reinhart G, Kopper R A, Maddox R P

机构信息

Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205.

出版信息

Nucleic Acids Res. 1987 Oct 26;15(20):8417-38. doi: 10.1093/nar/15.20.8417.

DOI:10.1093/nar/15.20.8417
PMID:3671087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306368/
Abstract

We have described the reaction of 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) with hen oviduct mRNA and have investigated the specific effects of AMT photoaddition on ovalbumin mRNA which constitutes 60-70% of oviduct mRNA. The photoreaction of AMT with hen oviduct mRNA appeared to occur in two phases - a rapid monoaddition followed by a slower conversion of monoadducts to diadducts (i.e. crosslinking). Both nondenaturing and denaturing gel electrophoresis revealed a photoreaction time dependent increase in ovalbumin mRNA electrophoretic mobility indicating the formation of a progressively more compact molecular structure. Identical analysis of photoreacted rabbit globin mRNA revealed no change in electrophoretic mobility suggesting that AMT was stabilizing the AU rich secondary structure of ovalbumin mRNA but having no similar effect on the relatively GC rich secondary structure of globin mRNA. Ovalbumin-specific DNA primer extension was used to demonstrate the selective and secondary structure specific photoaddition of AMT to uracil bases at the 5' end of ovalbumin mRNA.

摘要

我们已经描述了4'-氨甲基-4,5',8-三甲基补骨脂素（AMT）与母鸡输卵管mRNA的反应，并研究了AMT光加成对占输卵管mRNA 60-70%的卵清蛋白mRNA的特定影响。AMT与母鸡输卵管mRNA的光反应似乎分两个阶段进行——快速单加成，随后单加合物缓慢转化为双加合物（即交联）。非变性和变性凝胶电泳均显示，卵清蛋白mRNA电泳迁移率随光反应时间增加，表明形成了逐渐更紧密的分子结构。对光反应后的兔珠蛋白mRNA进行相同分析，结果显示电泳迁移率没有变化，这表明AMT稳定了卵清蛋白mRNA富含AU的二级结构，但对珠蛋白mRNA相对富含GC的二级结构没有类似影响。使用卵清蛋白特异性DNA引物延伸来证明AMT对卵清蛋白mRNA 5'端尿嘧啶碱基的选择性和二级结构特异性光加成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/f04fc9adbf6e/nar00264-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/6abae1a8022f/nar00264-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/c57737a9833d/nar00264-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/4a17a695f71f/nar00264-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/87a961679da3/nar00264-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/db6000bfe963/nar00264-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/03f31db4fac6/nar00264-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/d26dfe1a6116/nar00264-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/94d9730477a2/nar00264-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/f04fc9adbf6e/nar00264-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/6abae1a8022f/nar00264-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/c57737a9833d/nar00264-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/4a17a695f71f/nar00264-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/87a961679da3/nar00264-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/db6000bfe963/nar00264-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/03f31db4fac6/nar00264-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/d26dfe1a6116/nar00264-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/94d9730477a2/nar00264-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce1/306368/f04fc9adbf6e/nar00264-0311-a.jpg

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引用本文的文献

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3
The 9S RNA precursor of Escherichia coli 5S RNA has three structural domains: implications for processing.大肠杆菌5S RNA的9S RNA前体有三个结构域：对加工的启示。

本文引用的文献

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Characterization of the photoreaction between DNA and aminomethyl-trimethylpsoralen using absorption and fluorescence spectroscopy.
Photochem Photobiol. 1981 Jun;33(6):785-91. doi: 10.1111/j.1751-1097.1981.tb05493.x.
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Low-level psoralen--deoxyribonucleic acid cross-links induced by single laser pulses.
Biochemistry. 1981 Feb 17;20(4):739-45. doi: 10.1021/bi00507a012.
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Kinetics of formation of deoxyribonucleic acid cross-links by 4'-(aminomethyl)-4,5',8-trimethylpsoralen.4'-（氨甲基）-4,5',8-三甲基补骨脂素形成脱氧核糖核酸交联的动力学
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Identification of sites of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen cross-linking in Escherichia coli 23S ribosomal ribonucleic acid.4'-(羟甲基)-4,5',8-三甲基补骨脂素在大肠杆菌23S核糖体核糖核酸中的交联位点鉴定
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Marking the polarity of RNA molecules for electron microscopy by covalent attachment of psoralen-DNA restriction fragments.通过补骨脂素-DNA限制性片段的共价连接标记用于电子显微镜观察的RNA分子的极性
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Structure of E. coli 16S RNA elucidated by psoralen crosslinking.通过补骨脂素交联阐明的大肠杆菌16S RNA结构。
Cell. 1983 Apr;32(4):1355-65. doi: 10.1016/0092-8674(83)90316-1.
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Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of ribonucleic acid.4'-(羟甲基)-4,5',8-三甲基补骨脂素光加成反应对核糖核酸构象的依赖性
Biochemistry. 1982 Mar 16;21(6):1363-8. doi: 10.1021/bi00535a040.
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