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DNA复合物中A.T碱基对质子交换的选择性催化：亚氨基质子核磁共振分析

Selective catalysis of A.T base pair proton exchange in DNA complexes: imino proton NMR analysis.

作者信息

Strekowski L, Watson R A, Wilson W D

机构信息

Department of Chemistry, Georgia State University, Atlanta 30303.

出版信息

Nucleic Acids Res. 1987 Oct 26;15(20):8511-9. doi: 10.1093/nar/15.20.8511.

DOI:10.1093/nar/15.20.8511
PMID:3671090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306374/
Abstract

Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the selective exchange, involving transient opening and closing of individual A.T base pairs in the duplex, is discussed.

摘要

带有氨基链取代基的多芳族分子在与DNA结合后，会选择性地催化A.T碱基对质子与大量水质子的交换。胺催化的交换由对A.T和G.C碱基序列具有特异性的化合物、嵌入剂及外部结合剂介导。本文讨论了一种选择性交换机制，该机制涉及双链中单个A.T碱基对的瞬时打开和关闭。

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本文引用的文献

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Nature of the open state in long polynucleotide double helices: possibility of soliton excitations.长链多核苷酸双螺旋开放状态的本质：孤子激发的可能性。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7222-6. doi: 10.1073/pnas.77.12.7222.
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1H NMR relaxation studies of the hydrogen-bonded imino protons of poly(dA-dT).聚（dA-dT）中氢键合亚氨基质子的1H NMR弛豫研究
Biochemistry. 1984 Feb 28;23(5):944-55. doi: 10.1021/bi00300a023.
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Interactions of antitumor drugs with natural DNA: 1H NMR study of binding mode and kinetics.抗肿瘤药物与天然DNA的相互作用：结合模式和动力学的1H NMR研究
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A 300-MHz proton nuclear magnetic resonance investigation of deoxyribonucleic acid restriction fragments: dynamic properties.脱氧核糖核酸限制片段的300兆赫质子核磁共振研究：动态特性
Biochemistry. 1981 Jun 23;20(13):3764-9. doi: 10.1021/bi00516a015.
5
A 300 MHz and 600 MHz proton NMR study of a 12 base pair restriction fragment: investigation of structure by relaxation measurements.一个12碱基对限制片段的300兆赫和600兆赫质子核磁共振研究：通过弛豫测量对结构的研究。
Nucleic Acids Res. 1980 Dec 11;8(23):5795-812. doi: 10.1093/nar/8.23.5795.
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Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides.与质子和金属离子与核糖核酸、脱氧核糖核酸及其组成碱基、核苷和核苷酸相互作用相关的位点和热力学量。
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Mechanism of intercalation: ion effects on the equilibrium and kinetic constants for the interaction of propidium and ethidium with DNA.嵌入机制：离子对碘化丙啶和溴化乙锭与DNA相互作用的平衡常数和动力学常数的影响。
Biopolymers. 1985 Oct;24(10):1941-61. doi: 10.1002/bip.360241008.
8
A single mode of DNA base-pair opening drives imino proton exchange.单一模式的DNA碱基对打开驱动亚氨基质子交换。
Nature. 1987;328(6125):89-92. doi: 10.1038/328089a0.
9
Amplification of bleomycin-mediated degradation of DNA.博来霉素介导的DNA降解的扩增。
J Med Chem. 1987 Aug;30(8):1415-20. doi: 10.1021/jm00391a025.
10
Molecular basis for anticancer drug amplification: interaction of phleomycin amplifiers with DNA.抗癌药物扩增的分子基础：博来霉素扩增剂与DNA的相互作用
J Med Chem. 1986 Jul;29(7):1311-5. doi: 10.1021/jm00157a037.