DNA复合物中A.T碱基对质子交换的选择性催化：亚氨基质子核磁共振分析
Selective catalysis of A.T base pair proton exchange in DNA complexes: imino proton NMR analysis.
作者信息
Strekowski L, Watson R A, Wilson W D
机构信息
Department of Chemistry, Georgia State University, Atlanta 30303.
出版信息
Nucleic Acids Res. 1987 Oct 26;15(20):8511-9. doi: 10.1093/nar/15.20.8511.
Abstract
Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the selective exchange, involving transient opening and closing of individual A.T base pairs in the duplex, is discussed.
摘要
带有氨基链取代基的多芳族分子在与DNA结合后，会选择性地催化A.T碱基对质子与大量水质子的交换。胺催化的交换由对A.T和G.C碱基序列具有特异性的化合物、嵌入剂及外部结合剂介导。本文讨论了一种选择性交换机制，该机制涉及双链中单个A.T碱基对的瞬时打开和关闭。
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