Larson Eli J, Pergande Melissa R, Moss Michelle E, Rossler Kalina J, Wenger R Kent, Krichel Boris, Josyer Harini, Melby Jake A, Roberts David S, Pike Kyndalanne, Shi Zhuoxin, Chan Hsin-Ju, Knight Bridget, Rogers Holden T, Brown Kyle A, Ong Irene M, Jeong Kyowon, Marty Michael, McIlwain Sean J, Ge Ying
bioRxiv. 2023 Jan 3:2023.01.02.522513. doi: 10.1101/2023.01.02.522513.
Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite significant advances in nMS and TDP software developments, a unified and user-friendly software package for analysis of nTDP data remains lacking. Herein, we have developed MASH Native to provide a unified solution for nTDP to process complex datasets with database searching capabilities in a user-friendly interface. MASH Native supports various data formats and incorporates multiple options for deconvolution, database searching, and spectral summing to provide a one-stop shop for characterizing both native protein complexes and proteoforms. The MASH Native app, video tutorials, written tutorials and additional documentation are freely available for download at https://labs.wisc.edu/gelab/MASH_Explorer/MASHNativeSoftware.php . All data files shown in user tutorials are included with the MASH Native software in the download .zip file.
原生自上而下蛋白质组学（nTDP）将原生质谱（nMS）与自上而下蛋白质组学（TDP）相结合，以提供对蛋白质复合物的全面分析以及蛋白质异构体的鉴定和表征。尽管nMS和TDP软件开发取得了重大进展，但仍缺乏一个统一且用户友好的用于分析nTDP数据的软件包。在此，我们开发了MASH Native，为nTDP提供统一的解决方案，以便在用户友好的界面中通过数据库搜索功能处理复杂数据集。MASH Native支持各种数据格式，并结合了多种去卷积、数据库搜索和光谱求和选项，为表征原生蛋白质复合物和蛋白质异构体提供一站式服务。MASH Native应用程序、视频教程、书面教程和其他文档可在https://labs.wisc.edu/gelab/MASH_Explorer/MASHNativeSoftware.php上免费下载。用户教程中显示的所有数据文件都包含在下载的.zip文件中的MASH Native软件中。
