Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53705, United States.
Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53705, United States.
Bioinformatics. 2023 Jun 1;39(6). doi: 10.1093/bioinformatics/btad359.
Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite significant advances in nMS and TDP software developments, a unified and user-friendly software package for analysis of nTDP data remains lacking.
We have developed MASH Native to provide a unified solution for nTDP to process complex datasets with database searching capabilities in a user-friendly interface. MASH Native supports various data formats and incorporates multiple options for deconvolution, database searching, and spectral summing to provide a "one-stop shop" for characterizing both native protein complexes and proteoforms.
The MASH Native app, video tutorials, written tutorials, and additional documentation are freely available for download at https://labs.wisc.edu/gelab/MASH_Explorer/MASHSoftware.php. All data files shown in user tutorials are included with the MASH Native software in the download .zip file.
天然至上的蛋白质组学（nTDP）将天然质谱（nMS）与自上而下的蛋白质组学（TDP）相结合，提供对蛋白质复合物的全面分析，以及对蛋白质形式的鉴定和特征描述。尽管 nMS 和 TDP 软件的发展取得了重大进展，但仍然缺乏用于分析 nTDP 数据的统一且用户友好的软件包。
我们开发了 MASH Native，为 nTDP 提供了一个统一的解决方案，以便在用户友好的界面中使用数据库搜索功能处理复杂的数据集。MASH Native 支持各种数据格式，并结合了多种解卷积、数据库搜索和谱峰叠加选项，为同时对天然蛋白质复合物和蛋白质形式进行特征描述提供了一个“一站式”服务。
MASH Native 应用程序、视频教程、书面教程和其他文档可在 https://labs.wisc.edu/gelab/MASH_Explorer/MASHSoftware.php 免费下载。用户教程中显示的所有数据文件都包含在 MASH Native 软件的下载.zip 文件中。
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