Todor Horia, Herrera Nadia, Gross Carol
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA.
Lead Contact.
bioRxiv. 2023 Jan 5:2023.01.04.522824. doi: 10.1101/2023.01.04.522824.
Recent studies in bacteria suggested that the broadly conserved but enigmatic DedA proteins function as undecaprenyl-phosphate (UndP) flippases, recycling this essential lipid carrier. To determine whether all DedA proteins have UndP flippase activity, we performed a phylogenetic analysis and correlated it to previously published experimental results and predicted structures. We uncovered three major DedA subfamilies: one contains UndP flippases, the second contains putative phospholipid flippases and is associated with aerobic metabolism, and the third is found only in specific Gram-negative phyla.
DedA-family proteins are highly conserved and nearly ubiquitous integral membrane proteins found in Archaea, Bacteria, and Eukaryotes. Recent work revealed that eukaryotic DedA proteins are phospholipid scramblases and some bacterial DedA proteins are undecaprenyl phosphate flippases. We perform a phylogenetic analysis of this protein family in Bacteria revealing 3 DedA subfamilies with distinct phylogenetic distributions, genomic contexts, and putative functions. Our analysis lays the groundwork for a deeper understanding of DedA proteins and their role in maintaining and modifying the membrane.
最近在细菌中的研究表明，广泛保守但神秘的DedA蛋白作为磷酸十一异戊烯酯（UndP）翻转酶发挥作用，循环利用这种必需的脂质载体。为了确定所有DedA蛋白是否都具有UndP翻转酶活性，我们进行了系统发育分析，并将其与先前发表的实验结果和预测结构相关联。我们发现了三个主要的DedA亚家族：一个包含UndP翻转酶，第二个包含推定的磷脂翻转酶并与有氧代谢相关，第三个仅存在于特定的革兰氏阴性菌门中。
DedA家族蛋白是在古细菌、细菌和真核生物中发现的高度保守且几乎普遍存在的整合膜蛋白。最近的研究表明，真核生物的DedA蛋白是磷脂翻转酶，一些细菌的DedA蛋白是磷酸十一异戊烯酯翻转酶。我们对细菌中的这个蛋白家族进行了系统发育分析，揭示了具有不同系统发育分布、基因组背景和推定功能的3个DedA亚家族。我们的分析为更深入了解DedA蛋白及其在维持和修饰膜中的作用奠定了基础。
