Spatiotemporal based response for methylene blue removal using surface modified calcium carbonate microspheres coated with sp.

Calcium carbonate microspheres are attractive for their biocompatibility, high loading capacity and easy preparation. They can be used in biomedicine and catalytic applications. In the present work, calcium carbonate microspheres were surface modified with polyvinylpyrrolidone (PVP) followed by irradiation at 5 kGy prior to coating with sp. cells. To provide cell protection and internal energy storage, polyhydroxybutyrate (PHB) was induced using 3 factors 2 levels factorial design where the order of effect on PHB% was pH > incubation time > glucose concentration. The highest production was 81.68 PHB% at pH 9, 20 g L glucose and 4 days incubation time. sp. cells grown under PHB optimal conditions were used to coat the surface modified calcium carbonate microspheres. Characterization was performed using X-ray diffraction, Fourier Transform Infrared Spectroscopy, Dynamic light Scattering, Zeta potential and Scanning Electron Microscopy. The results obtained confirm the formation and coating of microspheres of 2.34 μm and -16 mV. The prepared microspheres were used in bioremoval of methylene blue dye, the results showed spatiotemporal response for MB-microsphere interaction, where PHB induced sp. coated microspheres initially adsorb MB to its outer surface within 1 h but decolorization takes place when the incubation time extends to 18 h. The microspheres can be reused up to 3 times with the same efficiency and with no desorption. These results suggest that the surface modified calcium carbonate can be tailored according to the requirement which can be delivery of biomaterial, bioadsorption or bioremediation.