Zhang Lujiao, Yang Yurun, Xiong Yan-Hua, Zhao Yu-Qing, Xiu Zongpeng, Ren Hui-Min, Zhang Kai, Duan Shun, Chen Ying, Xu Fu-Jian
State Key Laboratory of Chemical Resource Engineering, Key Lab of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Department of Orthopaedic Surgery, China-Japan Friendship Hospital, Beijing, 100029, China.
Bioact Mater. 2023 Jan 14;25:1-12. doi: 10.1016/j.bioactmat.2023.01.002. eCollection 2023 Jul.
The infections in open fracture induce high morbidity worldwide. Thus, developing efficient anti-infective orthopedic devices is of great significance. In this work, we designed a kind of infection-responsive long-term antibacterial bone plates. Through a facile and flexible volatilization method, a multi-aldehyde polysaccharide derivative, oxidized sodium alginate, was crosslinked with multi-amino compounds, gentamycin and gelatin, to fabricate a uniform coating on Ti bone plates via Schiff base reaction, which was followed by a secondary crosslinking process by glutaraldehyde. The double-crosslinked coating was stable under normal condition, and could responsively release gentamycin by the triggering of the acidic microenvironment caused by bacterial metabolism, owning to the pH-responsiveness of imine structure. The thickness of the coating was ranging from 22.0 μm to 63.6 μm. The coated bone plates (Ti-GOGs) showed infection-triggered antibacterial properties (>99%) and high biocompatibility. After being soaked for five months, it still possessed efficient antibacterial ability, showing its sustainable antibacterial performance. The anti-infection ability was demonstrated by an animal model of infection after fracture fixation (IAFF). At the early stage of IAFF, Ti-GOGs could inhibit the bacterial infection (>99%). Subsequently, Ti-GOGs could promote recovery of fracture of IAFF. This work provides a convenient and universal strategy for fabrication of various antibacterial orthopedic devices, which is promising to prevent and treat IAFF.
开放性骨折感染在全球范围内导致高发病率。因此，开发高效的抗感染骨科器械具有重要意义。在这项工作中，我们设计了一种感染响应型长效抗菌接骨板。通过一种简便灵活的挥发方法，将一种多醛多糖衍生物——氧化海藻酸钠与多氨基化合物、庆大霉素和明胶交联，通过席夫碱反应在钛接骨板上制备均匀涂层，随后通过戊二醛进行二次交联过程。双交联涂层在正常条件下稳定，由于亚胺结构的pH响应性，能够通过细菌代谢引起的酸性微环境触发而响应性释放庆大霉素。涂层厚度在22.0μm至63.6μm之间。涂覆的接骨板（Ti-GOGs）表现出感染触发的抗菌性能（>99%）和高生物相容性。浸泡五个月后，它仍具有高效的抗菌能力，显示出其可持续的抗菌性能。通过骨折固定后感染动物模型（IAFF）证明了其抗感染能力。在IAFF早期，Ti-GOGs可抑制细菌感染（>99%）。随后，Ti-GOGs可促进IAFF骨折的恢复。这项工作为制造各种抗菌骨科器械提供了一种方便通用的策略，有望用于预防和治疗IAFF。
