Lv Hongbin, Chen Zhen, Yang Xiaoping, Cen Lian, Zhang Xu, Gao Ping
School of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemical Technology, Beijing 100029, PR China.
J Dent. 2014 Nov;42(11):1464-72. doi: 10.1016/j.jdent.2014.06.003. Epub 2014 Jun 12.
Bacteria adhesion and subsequent biofilm formation are primary causes of implant associated infection. The biofilm makes the bacteria highly resistant to the host defense and antimicrobial treatment. Antibacterial coatings on the surface of titanium implant can prevent biofilm formation effectively, but it is still a challenge to accomplish relatively long lasting antibacterial effects before wound healing or formation of biological seal. The purpose of our work was to construct antibacterial multilayer coatings loaded with minocycline on surface of Ti substrates using chitosan and alginate based on layer-by-layer (LbL) self-assembly technique.
In this study, the surfaces of Ti substrates were first hydroxylated and then treated with 3-aminopropyltriethoxysilane (ATPES) to obtain amino-functionalized Ti substrates. Next, the precursor layer of chitosan was covalently conjugated to amino-functionalized Ti substrates. The following alternately coating alginate loaded with minocycline and chitosan onto the precursor layer of chitosan was carried out via LbL self-assembly technique to construct the multilayer coatings on Ti substrates.
The multilayer coatings loaded more minocycline and improved sustainability of minocycline release to kill planktonic and adherent bacteria. Moreover, surface charge and hydrophilicity of the coatings and antibacterial ability of chitosan itself also played roles in the antibacterial performance, which can keep the antibacterial ability of the multilayer coatings after minocycline release ceases.
In conclusion, LbL self-assembly method provides a promising strategy to fabricate long-term antibacterial surfaces, which is especially effective in preventing implant associated infections in the early stage.
Loading minocycline on the surface of implants based on LbL self-assembly strategy can endow implants with sustained antibacterial property. This can inhabit the immediate colonization of bacteria onto the surface of implants in the process of dental implant surgery, and thereby prevents and reduces the occurrence of periimplantitis.
细菌黏附及随后的生物膜形成是植入物相关感染的主要原因。生物膜使细菌对宿主防御和抗菌治疗具有高度抗性。钛植入物表面的抗菌涂层可有效防止生物膜形成，但在伤口愈合或生物密封形成之前实现相对持久的抗菌效果仍是一项挑战。我们研究的目的是基于层层（LbL）自组装技术，使用壳聚糖和海藻酸盐在钛基底表面构建负载米诺环素的抗菌多层涂层。
在本研究中，首先将钛基底表面羟基化，然后用3-氨丙基三乙氧基硅烷（ATPES）处理以获得氨基功能化的钛基底。接下来，壳聚糖的前体层与氨基功能化的钛基底共价结合。随后通过LbL自组装技术将负载米诺环素的海藻酸盐和壳聚糖交替涂覆在壳聚糖的前体层上，以在钛基底上构建多层涂层。
多层涂层负载了更多的米诺环素，并改善了米诺环素释放的可持续性以杀死浮游细菌和黏附细菌。此外，涂层的表面电荷和亲水性以及壳聚糖本身的抗菌能力在抗菌性能中也发挥了作用，这使得在米诺环素释放停止后多层涂层仍能保持抗菌能力。
总之，LbL自组装方法为制造长期抗菌表面提供了一种有前景的策略，这在早期预防植入物相关感染方面特别有效。
基于LbL自组装策略在植入物表面负载米诺环素可赋予植入物持续的抗菌性能。这可以在牙种植手术过程中抑制细菌立即定植在植入物表面，从而预防和减少种植体周围炎的发生。
